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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(03) [March 2006] >
| Title: | Constitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions |
| Authors: | Kumar, H
Prasad, S
Raj, J
Bhalla, T C |
| Keywords: | Acetonitrilase
Amidase
Nitrile hydratase
Nocardia globerula NHB-2 |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | Nocardia
globerula NHB-2 exhibited an intracellular
acetonitrile hydrolysing activity (AHA) when cultivated in nutrient broth
supplemented with glucose (10.0 g/l) and yeast extract (1.0 g/l), at pH 8.0, 30°C for 21 hr. Maximum AHA was recorded in the culture containing 0.1 M of sodium phosphate buffer, (pH 8.8) at 45°C for 15 min with 600 μmol of acetonitrile and resting cells of N. globerula NHB-2 equivalent to 1.0 ml
culture broth. This activity was stable up to 40°C and was completely inactivated at or above 60°C. About five-fold increase in AHA was observed after optimization
of culture and reaction conditions. Under the optimized conditions, this
organism hydrolyzed various nitriles and amides such as propionitrile,
benzonitrile, acetamide, and acrylamide to corresponding acids. This
nitrile/amide hydrolysing activity of N.
globerula NHB-2 has potential applications in enzymatic synthesis of
organic acids and bioremediation of nitriles and amides contaminated soil and
water system. |
| Page(s): | 240-245 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(03) [March 2006]
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