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IJEB Vol.44(03) [March 2006] >


Title: Optimum conditions for L-glutaminase production by actinomycete strain isolated from estuarine fish, Chanos chanos (Forskal, 1775)
Authors: Sivakumar, K
Sahu, Maloy Kumar
Manivel, P R
Kannan, L
Keywords: Actinomycetes
Chanos chanos
Estuarine fish
L-Glutaminase
Streptomyces rimosus
Issue Date: Mar-2006
Publisher: CSIR
Abstract: Actinomycetes were isolated from skin, gills and gut contents of estuarine fish, Chanos chanos using Kuster’s agar medium. Out of 20 strains tested, the strain LG-10 which was tentatively identified as Streptomyces rimosus showed L-glutaminase activity. Optimum production of L-glutaminase enzyme (17.51 IU/ml) was observed after 96 h of incubation at 27° C, pH 9 and glucose and malt extract as carbon and nitrogen sources, respectively. The present study indicated scope for the use of S. rimosus as an ideal organism for the industrial production of extracellular L-glutaminase.
Page(s): 256-258
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(03) [March 2006]

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