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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(03) [March 2006] >
| Title: | Optimum conditions for L-glutaminase production by actinomycete strain isolated from estuarine fish, Chanos chanos (Forskal, 1775) |
| Authors: | Sivakumar, K Sahu, Maloy Kumar Manivel, P R Kannan, L |
| Keywords: | Actinomycetes Chanos chanos Estuarine fish L-Glutaminase Streptomyces rimosus |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | Actinomycetes were isolated from skin, gills
and gut contents of estuarine fish, Chanos
chanos using Kuster’s agar medium. Out of 20 strains tested, the strain
LG-10 which was tentatively identified as Streptomyces
rimosus showed L-glutaminase activity. Optimum production of L-glutaminase
enzyme (17.51 IU/ml) was observed after 96 h of incubation at 27° C, pH 9 and glucose and
malt extract as carbon and nitrogen sources, respectively. The present study
indicated scope for the use of S. rimosus
as an ideal organism for the industrial production of extracellular
L-glutaminase. |
| Page(s): | 256-258 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(03) [March 2006]
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