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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(03) [March 2006] >
| Title: | In vitro cytogenetic studies of cypermethrin on human lymphocytes |
| Authors: | G, Suman
Naravaneni, Rambabu
Jamil, Kaiser |
| Keywords: | Chromosomal aberrations
Comet assay
Cypermethrin
Cytogenetic damage
Genotoxicity
In vitro methods
Metaphase
MTT assay |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | Assessment of cytotoxicity and response to
external factors like pesticides were evaluated by
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or MTT
assay, which measures mitochondrial metabolism in the entire cell culture and
provides information about the percentage of cell survival. Utilizing the MTT
assay, the cytotoxicity of cypermethrin was determined on lymphocyte cultures
from human peripheral blood samples, the short-term lymphocyte cultures were
incubated with various aliquots of the cypermethrin and the LC50 was
found to be 33.6  M. Lymphocytes treated with low-doses (1/10 of LC50) of
cypermethrin showed an increase in the frequency of chromosomal aberrations and
found to be significant. Karyotype analysis revealed more satellite
associations and chromosomal breaks in cypermethrin treated samples. Low-doses
of the pesticide also induced single-strand breaks in the DNA as assessed by
comet assay. The pesticide caused increase in the comet tail length with
increase in pesticide concentration, implicating genotoxicity in somatic cells.
It is concluded that In vitro assays
could give important information of the mechanism of toxicity at low dosages
and impact on genetic material of human origin. |
| Page(s): | 233-239 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(03) [March 2006]
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