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Title: In vitro cytogenetic studies of cypermethrin on human lymphocytes
Authors: G, Suman
Naravaneni, Rambabu
Jamil, Kaiser
Keywords: Chromosomal aberrations
Comet assay
Cytogenetic damage
In vitro methods
MTT assay
Issue Date: Mar-2006
Publisher: CSIR
Abstract: Assessment of cytotoxicity and response to external factors like pesticides were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or MTT assay, which measures mitochondrial metabolism in the entire cell culture and provides information about the percentage of cell survival. Utilizing the MTT assay, the cytotoxicity of cypermethrin was determined on lymphocyte cultures from human peripheral blood samples, the short-term lymphocyte cultures were incubated with various aliquots of the cypermethrin and the LC50 was found to be 33.6 M. Lymphocytes treated with low-doses (1/10 of LC50) of cypermethrin showed an increase in the frequency of chromosomal aberrations and found to be significant. Karyotype analysis revealed more satellite associations and chromosomal breaks in cypermethrin treated samples. Low-doses of the pesticide also induced single-strand breaks in the DNA as assessed by comet assay. The pesticide caused increase in the comet tail length with increase in pesticide concentration, implicating genotoxicity in somatic cells. It is concluded that In vitro assays could give important information of the mechanism of toxicity at low dosages and impact on genetic material of human origin.
Page(s): 233-239
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.44(03) [March 2006]

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