Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6408
Title: <i style="">In vitro</i> cytogenetic studies of cypermethrin on human lymphocytes
Authors: G, Suman
Naravaneni, Rambabu
Jamil, Kaiser
Keywords: Chromosomal aberrations
Comet assay
Cypermethrin
Cytogenetic damage
Genotoxicity
<i style="">In vitro</i> methods
Metaphase
MTT assay
Issue Date: Mar-2006
Publisher: CSIR
Abstract: Assessment of cytotoxicity and response to external factors like pesticides were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or MTT assay, which measures mitochondrial metabolism in the entire cell culture and provides information about the percentage of cell survival. Utilizing the MTT assay, the cytotoxicity of cypermethrin was determined on lymphocyte cultures from human peripheral blood samples, the short-term lymphocyte cultures were incubated with various aliquots of the cypermethrin and the LC<sub>50</sub> was found to be 33.6 <img src='/image/spc_char/micro.gif'><i style="">M</i>. Lymphocytes treated with low-doses (1/10 of LC<sub>50</sub>) of cypermethrin showed an increase in the frequency of chromosomal aberrations and found to be significant. Karyotype analysis revealed more satellite associations and chromosomal breaks in cypermethrin treated samples. Low-doses of the pesticide also induced single-strand breaks in the DNA as assessed by comet assay. The pesticide caused increase in the comet tail length with increase in pesticide concentration, implicating genotoxicity in somatic cells. It is concluded that <i style="">In vitro</i> assays could give important information of the mechanism of toxicity at low dosages and impact on genetic material of human origin.
Description: 233-239
URI: http://hdl.handle.net/123456789/6408
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(03) [March 2006]

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