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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(03) [March 2006] >
| Title: | Histopathological changes in experimental cholera with a non toxigenic non- O1 non-O139 Vibrio cholerae strain isolated from Kolkata, India |
| Authors: | Saha, D R
Pal, A
Rajendran, K
Dutta, P |
| Keywords: | Enterotoxic
Haemagglutinin protease
Inflammation
Non-O1 non- O139 Vibrio cholerae |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | This study was
conducted to understand the pathophysiological changes in experimental rabbit
ileal loop model using the Vibrio
cholerae strain non-O1non-O139, isolated as sole pathogen from clinically
diagnosed cholera patients in Kolkata. Significant amount of haemorrhagic fluid
accumulation was observed in all the test loops of rabbit model where the
strain of V.cholerae was inoculated
as compared to control loops. Microscopic examination of the accumulated fluid
showed the presence of erythrocytes and pus cells. Histology revealed
structural alteration of the villous epithelium with inflammatory cells
infiltration in all the layers of the gut mucosa including the nerve plexus region.
Preliminary observation with a haemagglutinin protease extracted from the
non-O1 non-O139 strain, was also studied in different concentrations in the
same animal model which showed similar type of macroscopic and microscopic
response in the ileal loops as seen with the original strain. The results
highlight that along with other pathways, inflammatory cells and the enteric
neurons have an important role in the pathophysiology of diarrhoea and the
isolated protease may be the probable virulence factor in initiating the
disease process in this non-O1non-O139 strain induced cholera. |
| Page(s): | 221-227 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(03) [March 2006]
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