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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(03) [March 2006] >
| Title: | Impaired cell motility in chronic myeloid leukemic granulocytes related to altered cytoskeletal pattern |
| Authors: | Kamble, M G
Advani, S H
Bhisey, A N |
| Keywords: | Bactericidal activity
Cell motility
Chronic myeloid leukemia
Cytoskeletal pattern
PMNL |
| Issue Date: | Mar-2006 |
| Publisher: | CSIR |
| Abstract: | The bactericidal
activity of polymorphonuclear leucocyte (PMNL) against infection stimulates
cytoskeletal changes accompanied with alteration in adhesion and locomotion.
Microfilaments, the motile apparatus is known to regulate these changes by
polymerization of monomeric G-actin to fibrous F-actin. PMNL from chronic
myeloid leukemia (CML) patients have been reported to be defective in
locomotion in response to synthetic peptide,
n-formyl-methionyl-leucyl-phenylalanine (fMLP) but the mechanism leading to
defective locomotion and their spatial reorganization remains unclear. Therefore,
in order to study the cause of defective motility of PMNL from CML patients the
spatial distribution and reorganization of microfilaments and microtubules in
response to fMLP have been examined by transmission electron (TEM) and scanning
electron microscopy (SEM). Under SEM, the PMNL-CML surface appeared smoother
with reduced ruffling resulting in rounding off cells with lesser polarized
morphology. Unstimulated PMNL from normal as well as CML subjects showed
shorter and fewer microtubules and evenly distributed microfilaments as
compared to fMLP stimulated PMNL. It is proposed that the cause of defective
locomotion was due to reduced surface activity as a consequence of altered
cytoskeletal configuration. This phenomenon seems to be related to impaired
functional appendages and as a whole led to the defective cell motility and
hence reduced chemotaxis in PMNL from CML patients. |
| Page(s): | 193-202 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(03) [March 2006]
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