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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(02) [February 2006] >
| Title: | Response surface optimization of effective medium constituents for the production of alkaline protease from a newly isolated strain of Pseudomonas aeruginosa |
| Authors: | Khan, Saif
Misra, A K
Tripathi, C K M
Mishra, B N
Bihari, Vinod |
| Keywords: | Central Composite Design
Response Surface Methodology
Proteases
Fermentation |
| Issue Date: | Feb-2006 |
| Publisher: | CSIR |
| Abstract: | Optimization of the fermentation
medium for maximum alkaline protease production was carried out with a new
strain of Pseudomonas aeruginosa (B-2).
Replacing the protein source/inducer (albumin in place of casein) brought about
significant increase in yield after 48 hr of inoculation. Three most effective
medium constituents identified by initial screening method of Plackett-Burman
were albumin, (NH4)2SO4 and glucose. Central
Composite Design (CCD) and Response Surface Methodology (RSM) were used in the
design of the experiment and in the analysis of the results. Optimum levels of
the effective medium constituents were albumin (6.586%); (NH4)2SO4,
0.164%; and glucose, 6.72%. The alkaline protease production increased
from 533460 to 793492 Ul-1. |
| Page(s): | 151-156 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(02) [February 2006]
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