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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(02) [February 2006] >
| Title: | Isolation and characterization of a new osmotolerant, non-virulent Klebsiella pneumoniae strain SAP for biosynthesis of succinic acid |
| Authors: | Thakker, Chandresh
Burhanpurwala, Zainab
Rastogi, Gurdeep
Shouche, Yogesh
Ranade, Dilip |
| Keywords: | Anaerobic bacteria
Klebsiella pneumoniae
Succinic acid |
| Issue Date: | Feb-2006 |
| Publisher: | CSIR |
| Abstract: | In the present study, isolation
of anaerobic bacteria from 24 different eco-niches was carried out. A total
number of 300 bacterial isolates, including 230 obligate and 70 facultative
anaerobes were obtained using anaerobic techniques. All the isolates were
initially screened for succinic acid production by Fluorescein test and TLC
method. During screening, 10 isolates found to produce succinic acid were
further examined by HPLC and then finally confirmed for succinic acid by LC-MS
analysis. Amongst 10 isolates, isolate SAP, a facultative anaerobe isolated
from buffalo rumen fluid, showed maximum yield of 2.1 g/l of succinic acid from
10 g of glucose in 24 hr under anaerobic condition. This isolate was identified
as Klebsiella pneumoniae strain SAP
by 16S rDNA sequence and signature sequence analysis. Mouse lethality test for
the strain SAP showed LD50 value of 3.3 × 108 CFU/ml,
which shows non-virulent nature of the strain. This strain may become a
candidate strain for succinic acid production because of its osmotolerant
nature and higher succinate:acetate ratio. |
| Page(s): | 142-150 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(02) [February 2006]
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