Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6364
Title: Binding of diclofenac sodium with bovine serum albumin at different temperatures, <i style="">p</i>H and ionic strengths
Authors: Dutta, S K
Basu, S K
Sen, K K
Keywords: Albumin
Bovine serum
Diclofenac sodium
Ionic strength
Issue Date: Feb-2006
Publisher: CSIR
Abstract: The study was designed to examine the binding of diclofenac sodium with bovine serum albumin (BSA) at different temperatures (20°, 30° and 40°C),<sup> </sup><i style="">p</i>H (6.4,7.4 and 8.4) and ionic strengths (<img src='/image/spc_char/micro.gif'> = 0.1, 0.2 and 0.3) by means of equilibrium dialysis method. The concentration of diclofenac sodium was maintained at wider range from 15 to 900 <img src='/image/spc_char/micro.gif'>mole/l and BSA concentration was maintained at 61.5 <img src='/image/spc_char/micro.gif'>mole/l. The data obtained were interpreted by nonlinear regression method using Graphpad prism software. The analysis showed that the interaction between diclofenac sodium with BSA results in two-site saturable binding. A decrease in association constant was observed with increasing temperature. The average standard free energy change (ΔG°) value was –7.07 (site I) and –4.2 (site II) Kcal/mol. The standard enthalpy change (ΔH°) and the standard entropy change (ΔS°) were–7.8 Kcal/mole, –2.35 cal/mole (site I) and –7.4 Kcal/mole, –10.5 cal/mole (site II), respectively. The negative enthalpy change suggested the binding between diclofenac sodium and the binding sites of BSA were spontaneous and exothermic. The negative value of ΔH° and ΔS° indicated hydrogen bonding and van der Waal’s force was the major mechanism for diclofenac sodium and BSA interaction. Increase in <i style="">p</i>H and ionic strength also caused decrease in association constant of diclofenac sodium and BSA binding.
Description: 123-127
URI: http://hdl.handle.net/123456789/6364
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(02) [February 2006]

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