|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(02) [February 2006] >
| Title: | Methylation of E-cadherin and hMLH1 genes in Indian sporadic breast carcinomas |
| Authors: | Viswanathan, M
Solomon, S P R
Tsuchida, N
Selvam, G S
Shanmugam, G |
| Keywords: | Breast carcinoma
E-cadherin
hMLH1 gene
Indian sporadic carcinoma
Methylation |
| Issue Date: | Feb-2006 |
| Publisher: | CSIR |
| Abstract: | Hypermethylation of promoter regions leading to inactivation
of tumor suppressor genes is a common event in the progression of several tumor
types. We have employed a novel restriction digestion based multiplex PCR assay
to analyse the methylation status of promoter regions of tumor suppressor genes
(p16, hMLH1, MGMT and E-cadherin) in sporadic breast carcinomas of Indian women. The present
results indicated the absence of hypermethylation in promoter region of p16 and MGMT genes. However,
6 of the 19 (31.6%) sporadic breast carcinomas showed hypermethylation in the
promoters of two of the genes analysed; three in hMLH1 and another three in
E-cad. Since our earlier studies have shown lack of genetic
alterations such as missense mutations and deletions in the tumor associated
genes-p16, ras and p14ARF in sporadic breast
tumors, the epigenetic alterations of the two genes reported in the present
study could be of interest and might be among the events in the
genesis/progression of sporadic breast carcinomas. |
| Page(s): | 115-119 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(02) [February 2006]
|
|
