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|Title:||Studies on somatic cell variability in <i>Commiphora wightii</i> (Arnott.) Bhandari for guggulsterone production|
Ramawat, K G
Somatic cell variability
|Series/Report no.:||Int. cl.<sup>8</sup> — A61K 36/00, A01G 31/00|
|Abstract:||More than 125 clones were established in <b><i>Commiphora wightii </i></b><b>(Arnott.) Bhandari</b> by aggregate cloning from stock cultures maintained on MS medium supplemented with 0.25 mg/l 2,4,5-trichlorophenoxyacetic acid and 0.1 mg/l kinetin. A few high productive clones were isolated while majority (>80%) were low productive. In the present paper, the somatic cell variability in callus cultures of <b><i>C. wightii </i></b>and their growth in shake flasks and bioreactor for high yield of guggulsterones has been reported. A high productive clone yielded 80μg/l guggulsterones when grown in cell suspension culture in shake flasks and 2 litre stirred tank bioreactor. The colour variations of the cultures (due to anthocyanin accumulation) were also explored to isolate high guggulsterone producing lines; however, no correlationship could be established between anthocyanin accumulation and guggulsterone contents of the clones.|
|Appears in Collections:||NPR Vol.8(5) [September-October 2009]|
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