NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)]
 >
NPR Vol.8 [2009] >
NPR Vol.8(5) [September-October 2009] >


Title: Studies on somatic cell variability in Commiphora wightii (Arnott.) Bhandari for guggulsterone production
Authors: Dass, Suchismita
Ramawat, K G
Keywords: Anthocyanin
Callus culture
Cell culture
Commiphora wightii
Guggulsterone
Somatic cell variability
Issue Date: Oct-2009
Publisher: CSIR
IPC CodeInt. cl.8 — A61K 36/00, A01G 31/00
Abstract: More than 125 clones were established in Commiphora wightii (Arnott.) Bhandari by aggregate cloning from stock cultures maintained on MS medium supplemented with 0.25 mg/l 2,4,5-trichlorophenoxyacetic acid and 0.1 mg/l kinetin. A few high productive clones were isolated while majority (>80%) were low productive. In the present paper, the somatic cell variability in callus cultures of C. wightii and their growth in shake flasks and bioreactor for high yield of guggulsterones has been reported. A high productive clone yielded 80μg/l guggulsterones when grown in cell suspension culture in shake flasks and 2 litre stirred tank bioreactor. The colour variations of the cultures (due to anthocyanin accumulation) were also explored to isolate high guggulsterone producing lines; however, no correlationship could be established between anthocyanin accumulation and guggulsterone contents of the clones.
Page(s): 532-536
ISSN: 0975-1092(Online); 0972-592X(Print)
Source:NPR Vol.8(5) [September-October 2009]

Files in This Item:

File Description SizeFormat
NPR 8(5) 532-536.pdf431.9 kBAdobe PDFView/Open
 Current Page Visits: 472 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 513178 since 06-Feb-2009  Last updated on 21-Apr-2014Webmaster: nopr@niscair.res.in