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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(01) [January 2006] >
| Title: | In vitro clonal propagation of Chlorophytum borivilianum Sant. et Fernand., a rare medicinal herb from immature floral buds along with inflorescence axis |
| Authors: | Sharma, Urvashi
Mohan, J S S |
| Keywords: | Aphrodisiac
Fasciculated roots
Rare species
Safed musli
Shoot multiplication |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| Abstract: | A novel method of shoot regeneration from
immature floral buds along with inflorescence axis in C. borivilianum, a rare medicinal herb is described. Using this
explant, axenic cultures were established with very less contamination (10%).
MS medium with 2 mg l-1 kinetin and 0.1 mg l-1 2,
4-dichlorophenoxyacetic acid proved to be the best for multiple shoot
induction. Maximum number (35) of shoot production was achieved in MS medium
with 2 mg l-1 benzylaminopurine. Rooting of shoots (86.7%) with
maximum fasciculated roots (5) occurred on Knops medium containing iron and
vitamins of MS medium with 2 mg l-1 indole-3-butyric acid and 0.1%
activated charcoal. Plant survival was 80% in four weeks after their removal
from in vitro conditions. Per explant
34 hardened plants generated within 50 weeks. This protocol can be useful for
large-scale clonal multiplication from immature floral buds with inflorescence
axis and successfully used for germplasm conservation of this rare medicinal
herb without destroying the mother plant. |
| Page(s): | 77-82 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(01) [January 2006]
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