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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(01) [January 2006] >
| Title: | Intraspecific strains of Pythium aphanidermatum induced disease resistance in ginger and response of host proteins |
| Authors: | Ghosh, Rajyasri
Datta, Moumita
Purkayastha, R P |
| Keywords: | Defence activator
Defence proteins
Ginger
Induced resistance
Intraspecific strains
Pythium aphanidermatum
Rhizome rot |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| Abstract: | Intraspecific strains of Pythium aphanidermatum induced
resistance in ginger against rhizome rot and activated biosynthesis of selected
host proteins. Pre-inoculation of plants with IR strain (avirulent) or
co-inoculation with SR2 (virulent) caused significant reduction in
disease severity. Analysis of protein profiles of ginger leaves of inoculated
and non-inoculated plants by SDS-PAGE and Image Master VDS-ID Gel Analysis
version : 3.0 revealed that some specific defence proteins/stress proteins
increased in inoculated plants. Five such proteins having molecular weight 56,
32, 27, 18 and 14 kDa were detected in leaves of plant treated with IR + SR2
strains. On the contrary, mycelial protein profiles and submerged growth
of strains were studied separately and together. Mycelia of IR, SR2
and IR + SR2 exhibited 26, 23 and 25 protein bands, respectively
although, 21 bands were common between IR and SR2. Growth of SR2
in synthetic medium was much higher than that of IR, but the growth of two
strains together was lower than SR2 alone. To characterise strains,
their differential growth response to DL- -aminobutyric acid (BABA), a known defence activator of ginger was
also tested. Results suggested that at least 5 specific defence proteins/stress
proteins were involved in microbially induced resistance in ginger and inducer
strains were distinct in their specific protein profiles and sensitivity to
BABA. |
| Page(s): | 68-72 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(01) [January 2006]
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