Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6340
Title: Intraspecific strains of <i style="">Pythium aphanidermatum</i> induced disease resistance in ginger and response of host proteins
Authors: Ghosh, Rajyasri
Datta, Moumita
Purkayastha, R P
Keywords: Defence activator
Defence proteins
Ginger
Induced resistance
Intraspecific strains
<i style="">Pythium aphanidermatum</i>
Rhizome rot
Issue Date: Jan-2006
Publisher: CSIR
Abstract: Intraspecific strains of <i style="">Pythium aphanidermatum</i> induced resistance in ginger against rhizome rot and activated biosynthesis of selected host proteins. Pre-inoculation of plants with IR strain (avirulent) or co-inoculation with SR<sub>2</sub> (virulent) caused significant reduction in disease severity. Analysis of protein profiles of ginger leaves of inoculated and non-inoculated plants by SDS-PAGE and Image Master VDS-ID Gel Analysis version : 3.0 revealed that some specific defence proteins/stress proteins increased in inoculated plants. Five such proteins having molecular weight 56, 32, 27, 18 and 14 kDa were detected in leaves of plant treated with IR + SR<sub>2 </sub>strains. On the contrary, mycelial protein profiles and submerged growth of strains were studied separately and together. Mycelia of IR, SR<sub>2</sub> and IR + SR<sub>2</sub> exhibited 26, 23 and 25 protein bands, respectively although, 21 bands were common between IR and SR<sub>2</sub>. Growth of SR<sub>2</sub> in synthetic medium was much higher than that of IR, but the growth of two strains together was lower than SR<sub>2</sub> alone. To characterise strains, their differential growth response to DL-<img src='/image/spc_char/beta.gif'>-aminobutyric acid (BABA), a known defence activator of ginger was also tested. Results suggested that at least 5 specific defence proteins/stress proteins were involved in microbially induced resistance in ginger and inducer strains were distinct in their specific protein profiles and sensitivity to BABA.
Description: 68-72
URI: http://hdl.handle.net/123456789/6340
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(01) [January 2006]

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