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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(01) [January 2006] >
| Title: | In vitro antioxidant activity of Diospyros malabarica Kostel bark |
| Authors: | Mondal, Susanta Kumar
Chakraborty, Goutam
Gupta, M
Mazumder, U K |
| Keywords: | Antioxidant
Diospyros malabarica
Free radicals
Phenolic compound
Reductive ability |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| Abstract: | Antioxidant
activity of defatted methanol extract of D.
malabarica bark was studied for its free radical scavenging property on
different in vitro models e.g.
1,1-diphenyl-2-picryl hydrazyl (DPPH), nitric oxide, superoxide, hydroxyl
radical and lipid peroxide radical model. The extract showed good
dose-dependent free radical scavenging property in all the models except in
hydroxyl radical inhibition assay. IC50 values were found to be
9.16, 13.21, 25.27 and 17.33 µg/ml respectively in DPPH, nitric oxide,
superoxide and lipid peroxidation inhibition assays. In hydroxyl radical
inhibition assay 1000 µg/ml extract showed only 10% inhibition with respect to
the control. Measurement of total phenolic compounds by Folin-Ciocalteu’s
phenol reagent indicated that 1mg of the extract contained 120.7µg equivalent
of pyrocatechol. The results indicate that the antioxidant property of the
extract may be due to the high content of phenolic compounds. However, the
underlying mechanism may not involve hydroxyl radical scavenging property. |
| Page(s): | 39-44 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(01) [January 2006]
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