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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(01) [January 2006] >
| Title: | Studies on diagnostic potential of secretory antigens of Mycobacterium bovis |
| Authors: | Trivedi, Shweta
Das, S K
Kumar, Rajesh
Chandra, Rajesh |
| Keywords: | : Culture filtrate proteins
Delayed type hypersensitivity
Mycobacterium bovis
Purified protein derivative |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| Abstract: | Culture filtrate proteins (CFPs) of M. bovis were produced by culturing the
organism for a period of 3 weeks. These CFPs elicited a good delayed
hypersensitivity (DTH) reaction in cattle and guinea pig. However, CFPs showed
least cross–reactivity when tested in guinea pigs sensitized with M. bovis BCG and M. phlei. CFPs were found to be well recognized by T–cells of
single intradermal test (SIDT) positive cattle and were also sero-reactive.
Lymphocyte transformation assay revealed the culture filtrate antigens as major
T-cell antigens. CFPs induced a good interferon gamma release as
compared to conventional antigen, PPD, thereby highlighting their diagnostic
capabilities. |
| Page(s): | 14-18 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(01) [January 2006]
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