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Title: Effect of C-terminal 44 amino acids deletion on activity of Haemophilus influenzae UvrA protein
Authors: Kulkarni, Amit S
Khalap, Nutan
Joshi, Vasudha P
Keywords: C-terminal deletion;DNA binding;Haemophilus influenzae;Nucleotide Excision Repair;UvrAΔC44 protein
Issue Date: Jan-2006
Publisher: CSIR
Abstract: UV-sensitive mutant strain of Haemophilus influenzae Rd MBH3, is 20 times more sensitive to UV irradiation than the wild type strain. The mutation responsible for increased UV sensitivity of the strain was identified as G→A transition predicting synthesis of truncated UvrAΔC44 protein (Balsara & Joshi)12. Recombinant UvrAΔC44 protein was purified for the first time under denaturing conditions. The molecular weight of the recombinant protein was estimated as ~100 kDa. Recombinant UvrAΔC44 protein was found to be less efficient in its ATPase and DNA binding activity as compared to the wild type protein. Recombinant plasmid carrying uvrAΔC44 gene could partially complement the UvrA deficiency in E. coli UvrA mutant.
Page(s): 7-13
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(01) [January 2006]

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