|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.44 [2006] >
IJEB Vol.44(01) [January 2006] >
| Title: | Effect of C-terminal 44 amino acids deletion on activity of Haemophilus influenzae UvrA protein |
| Authors: | Kulkarni, Amit S
Khalap, Nutan
Joshi, Vasudha P |
| Keywords: | C-terminal deletion
DNA binding
Haemophilus influenzae
Nucleotide Excision Repair
UvrAΔC44 protein |
| Issue Date: | Jan-2006 |
| Publisher: | CSIR |
| Abstract: | UV-sensitive mutant strain of Haemophilus influenzae Rd MBH3, is 20
times more sensitive to UV irradiation than the wild type strain. The mutation
responsible for increased UV sensitivity of the strain was identified as G→A transition predicting synthesis of truncated UvrAΔC44 protein
(Balsara & Joshi)12. Recombinant UvrAΔC44 protein was purified
for the first time under denaturing conditions. The molecular weight of the
recombinant protein was estimated as ~100 kDa. Recombinant UvrAΔC44 protein was
found to be less efficient in its ATPase and DNA binding activity as compared
to the wild type protein. Recombinant plasmid carrying uvrAΔC44 gene could partially complement the UvrA deficiency in E. coli UvrA mutant. |
| Page(s): | 7-13 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.44(01) [January 2006]
|
|
