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Title: Effect of C-terminal 44 amino acids deletion on activity of <i style="">Haemophilus influenzae</i> UvrA protein
Authors: Kulkarni, Amit S
Khalap, Nutan
Joshi, Vasudha P
Keywords: C-terminal deletion
DNA binding
Haemophilus influenzae
Nucleotide Excision Repair
UvrAΔC44 protein
Issue Date: Jan-2006
Publisher: CSIR
Abstract: UV-sensitive mutant strain of <i style="">Haemophilus influenzae</i> Rd MBH3, is 20 times more sensitive to UV irradiation than the wild type strain. The mutation responsible for increased UV sensitivity of the strain was identified as G→A transition predicting synthesis of truncated UvrAΔC44 protein (Balsara & Joshi)<sup>12</sup>. Recombinant UvrAΔC44 protein was purified for the first time under denaturing conditions. The molecular weight of the recombinant protein was estimated as ~100 kDa. Recombinant UvrAΔC44 protein was found to be less efficient in its ATPase and DNA binding activity as compared to the wild type protein. Recombinant plasmid carrying <i style="">uvr</i>AΔC44 gene could partially complement the UvrA deficiency in <i style="">E. coli</i> UvrA mutant.
Description: 7-13
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.44(01) [January 2006]

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