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Title: Optimization of acid hydrolysis conditions of delimed tannery fleshings by response surface method
Authors: Rai, Amit Kumar
Nived, C
Sakhare, P Z
Suresh, P V
Bhaskar, N
Mahendrakar, N S
Keywords: Antioxidant;Optimization;Organic acids;Protein hydrolysate;Tannery fleshings
Issue Date: Nov-2009
Publisher: CSIR
Abstract: An ecofriendly method, involving mild organic acids to hydrolyse delimed tannery fleshings (TF), has been developed. Combination of formic acid and propionic acid (1:1) was employed to obtain hydrolysates with higher degree of hydrolysis (DH). Better antioxidant properties were obtained by response surface method (RSM) using a factorial design. Effect of pretreatment involving steam cooking (80°C for 15 min) indicated that cooked delimed TF on acid treatment had significantly (P<0.5) higher protein extractability than uncooked delimed TF. Optimized levels of factors for obtaining highest DH (>51%) at room temperature for cooked delimed TF were acid mixture (20%) and incubation time (9 days). Liquid hydrolysate from TFs exhibited antioxidant activity (125.56 g ascorbic acid equivalents /ml extract) and diphenyl picrylhydrazyl (DPPH) radical scavanging activity (46.42%). Chemical score of hydrolysate revealed an excess amount of essential amino acids (arginine, leucine and lysine) as compared to reference protein. This indicates its potential for use in livestock/aquaculture feeds.
Page(s): 967-974
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.68(11) [November 2009]

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