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Title: Effect of gallic acid on alkaline phosphatase and peptidase activities in rat intestine
Authors: Mahajan, Nidhi
Mahmood, Akhtar
Keywords: Leucine aminopeptidase
-Glutamyl transpeptidase
Intestinal alkaline phosphatase
Enzyme inhibition
Gallic acid
Rat intestine
Brush border membrane
Issue Date: Oct-2009
Publisher: CSIR
Abstract: Gallic acid is a normal constituent of many edible foods, thus directly interacts with epithelial tissue in intestine. In the present study, the effect of gallic acid on intestinal alkaline phosphatase (IAP) and peptidase activities in rat intestine was evaluated. Gallic acid (0.27-0.5 mM) inhibited activities of leucine aminopeptidase (LAP) and -glutamyl transpeptidase (-GTP) by over 90%, compared to controls in rat intestine. In contrast, 0.1-0.6 mM gallic acid either had no effect or stimulated the activity of IAP in rat intestine. The observed inhibition of peptidases by gallic acid was reversible in nature. Kinetic analysis revealed no change in Vmax of LAP (0.42-0.44 units/mg protein) and -GTP (0.22-0.24 units/mg protein), while the values of apparent Km were increased 6-7 fold, exhibiting competitive-type of enzyme inhibition by gallic acid. The values of Ki for LAP and -GTP were 0.037 mM and 0.017 mM, respectively. These observations indicate that gallic acid is a potent inhibitor of brush border peptidases, and thus may interfere in the digestion and absorption of proteins in the intestine.
Page(s): 378-382
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.46(5) [October 2009]

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