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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(5) [October 2009] >
| Title: | Protective role of Cassia auriculata leaf extract on hyperglycemia-induced oxidative stress and its safety evaluation |
| Authors: | Gupta, Shipra
Sharma, Suman Bala
Prabhu, Krishna Madhava
Bansal, Surendra Kumar |
| Keywords: | Cassia auriculata
Diabetes mellitus
Streptozotocin
Phytochemicals
Antioxidant activity
Toxicity assessment |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | Cassia auriculata L.
(Caesalpiniaceae) is widely used from the ancient period to treat diabetes
mellitus. In the present study, the antioxidant activity of C. auriculata aqueous leaf extract
(CLEt) was evaluated in streptozotocin-induced mild diabetic (MD) and severe
diabetic (SD) rats. A short-term toxicity assessment was also conducted in
healthy rats to examine toxic effects of the extract. Oral administration of
CLEt to MD and SD rats (100, 200 and 400 mg/kg body weight per day for a period
of 21 days) produced significant fall in fasting blood glucose (FBG) in a
dose-dependent manner. Treatment with the extract (400 mg/kg) showed
significant reduction in serum levels of thiobarbituric acid reactive
substances (TBARS) and oxidized low-density lipoprotein (OxLDL) in both MD and
SD rats. The antioxidant defense system was also found to be improved in
CLEt-treated (400 mg/kg) MD and SD rats, as revealed by significant increase in
activities of erythrocyte’s antioxidant enzymes i.e. superoxide dismutase (SOD)
and catalase (CAT) with a concomitant elevation in erythrocyte’s reduced
glutathione (GSH) content. Moreover, there were no toxic signs in rats treated
with high doses of the extract (1000 and 2000 mg/kg body weight per day for 21
days). Blood glucose, hepatic and renal function parameters in these rats were
found within normal limits. Phytochemical screening of CLEt revealed the
presence of alkaloids, flavonoids, saponins, tannins and cardiac glycosides
with antihyperglycemic and antioxidant properties. This study suggests that
CLEt possesses potent antioxidant activity along with antihyperglycemic
potential, hence protective against diabetic complications. |
| Page(s): | 371-377 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.46(5) [October 2009]
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