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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(5) [October 2009] >
| Title: | Efficient and rapid purification of lentil  -galactosidase by affinity precipitation with alginate |
| Authors: | Çelem, Evran Bıçak
Bolle, Sharon Sibel
Önal, Seçil |
| Keywords: | Affinity macroligand
Affinity precipitation
Alginate
-Galactosidase
Lentil seed
Purification |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | -Galactosidase (-D-galactoside galactohydrolase, EC 3.2.1.22) was purified (26-fold) from the germinating seeds of lentil (Lens culinaris) by affinity precipitation with alginate. The purified enzyme gave a single band corresponding to molecular mass of 40 kDa on SDS-PAGE. The optimum temperature and pH of the enzyme were determined as 40oC and 5.5, respectively. The enzyme was very stable at a temperature range of 4-65oC and at a pH range of 4-7. The values of kinetic constants Km and Vmax using p-nitrophenyl--D-galactopyranoside (PNPG) as substrate were 0.191 mM and 0.73 U, respectively. Results suggest that affinity precipitation is an attractive process for the purification of -galactosidase. |
| Page(s): | 366-370 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.46(5) [October 2009]
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