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Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.16 [2009] >
IJCT Vol.16(5) [September 2009] >
| Title: | Partial purification and characterization of a highly thermostable and pH stable endoglucanase from a newly isolated Bacillus strain M-9 |
| Authors: | Bajaj, Bijender K
Pangotra, Himani
Wani, Masood A
Sharma, Priyanka
Sharma, Ajay |
| Keywords: | Endoglucanase
Bacillus strain M-9
Thermostable
Acid/alkalistable |
| Issue Date: | Sep-2009 |
| Publisher: | CSIR |
| Abstract: | Cellulases to be
employed for lignocellulose bioconversion for biofuel (ethanol) industry or for
production of other specialty chemicals must be extremely robust to withstand
harsh industrial conditions like extremes of temperature, pH, presence
of inhibitory agents etc. In the present study the Bacillus strain M-9 isolated from decomposing rice bran produced
significant amount of endoglucanase after 72 h of fermentation. Endoglucanase
was partially purified by ammonium sulphate precipitation (20-50% saturation)
and characterized for few properties. The endoglucanase showed activity over
broad range of temperatures (30-100oC) with maximum activity at 60oC.
The enzyme retained 50 and 39% of the maximum activity at 90 and 100oC,
respectively. The enzyme was thoroughly stable at 50-70oC for 30
min-1 h. The endoglucanase showed activity over a wide range of pH (3-10)
with maximum activity at pH 5. Detergents (SDS, Tween 80), metal ions
(Zn2+, Pb2+) and EDTA caused little to moderate reduction
in enzyme activity. The endoglucanase was purified to the extent of 9.06 folds
by salt precipitation and DEAE-cellulose chromatography. Endoglucanase showed
molecular weight of approximately 54 kDa as examined by SDS-PAGE. High
thermostability and acid/alkalistability of the endoglucanase reflect potential
commercial significance of the enzyme. |
| Page(s): | 382-387 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.16(5) [September 2009]
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