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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >
| Title: | Retroviral vectors and gene therapy: An update |
| Authors: | Maurya, S K
Srivastava, Sushant
Joshi, R K |
| Keywords: | Gene therapy
retroviruses
vector |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | Gene therapy aims at treatment of diseases by transfer of genetic material
into specific cells of a patient. So the transduction of appropriate target
cell is critical. Retroviruses infect nearly every cell in the target
population and become integrated in host cell genome for a stable expression.
All retroviruses have only three structural genes gag, pol and env,
except for lentiviruses where two other genes tat, rev and four
accessory genes are required (vif, vpu, nif, vpr). There are two
components of retroviral vector system—i) Packaging cell lines, which provide
the products of gag, pol & env genes but are unable to
package itself as they lack the  sequence, and ii) retroviral
vectors where gag, pol and env are deleted but sequence along with LTR is present. Several other types of vectors are
also described. |
| Page(s): | 349-357 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(4) [October 2009]
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