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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >
| Title: | Assessment of genetic diversity among Podophyllum hexandrum genotypes of the North-western Himalayan region for podophyllotoxin production |
| Authors: | Alam, Md Afroz
Gulati, Pallavi
Gulati, Aswini K
Mishra, Gyan P
Naik, Pradeep K |
| Keywords: | Podophyllum hexandrum
podophyllotoxin
genetic diversity
RAPD |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: |
Podophyllum hexandrum (Indian Mayapple) is an important medicinal
plant valued all over the world. Genetic diversity among the 28 genotypes of P. hexandrum distributed in 11
geographical regions from Himachal Pradesh (a part of the North-western Himalaya) was analyzed using RAPD markers. The genetic
diversity was high among the genotypes as measured by percentage of polymorphic
bands (PPB = 92.37%) and Shanon information index (I = 0.50). The mean
coefficient of gene differentiation (Gst) was 0.69, indicating that 33.77% of the
genetic diversity resided within the genotypes. Analysis of molecular variance
indicated that the source of variation among the groups was 53% and among the
genotypes of groups was 47%. An overall value of mean estimated number of gene
flow (Nm = 0.22) indicated that there was limited gene flow among the genotypes.
The existence of variation among the 28 genotypes as observed through
podophyllotoxin content proved to be coupled with geographical altitude (r =
0.92) and local ecological conditions (temperature, rainfall, humidity, soil pH, etc.) but not on genetic basis (r =
-0.55). Based on the observed genetic variations among the genotypes of Podophyllum, we recommend for their in situ conservation and germplasm
collection expeditions in future conservation plans. |
| Page(s): | 391-399 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(4) [October 2009]
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