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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >
| Title: | Micropropagation of Dendrobium transparens L. from axenic pseudobulb segments |
| Authors: | Sunitibala, H
Kishor, Rajkumar |
| Keywords: | Axenic pseudobulb segment
benzyl aminopurine
Dendrobium transparens
in vitro
microshoot |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | An efficient protocol for micropropagation of Dendrobium transparens L. using the axenic pseudobulb segments,
derived from in vitro germinated
seedlings, was developed. The immature embryos from 120-d-old capsules after
pollination were first germinated on half-strength Murashige and Skoog (MS)
basal medium supplemented with 1 mg L-1 of naphthalene acetic acid
(NAA) + 2 mg L-1 of benzyl aminopurine (BAP). For multiple shoot
induction, the axenic nodal segments excised from 120-d-old seedlings were
cultured on the medium containing different concentrations and combinations of
NAA and BAP. The multiplied shoots were then transferred to half-strength MS
medium supplemented with 1 mg L-1 of indole-3 acetic acid (IAA) or indole-3
butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved
when the medium was supplemented with 1 mg L-1 NAA and 2 mg L-1
BAP, while that for rooting with 1 mg L-1 IAA. The plantlets
were hardened off and more than 90% of survived plants were released to the
shade house. The plantlets flowered 1 yr after transplantation |
| Page(s): | 448-452 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(4) [October 2009]
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