Please use this identifier to cite or link to this item:
|Title:||Micropropagation of <i style="">Dendrobium transparens</i> L. from axenic pseudobulb segments|
|Keywords:||Axenic pseudobulb segment|
|Abstract:||An efficient protocol for micropropagation of <i style="">Dendrobium transparens</i> L. using the axenic pseudobulb segments, derived from <i style="">in vitro</i> germinated seedlings, was developed. The immature embryos from 120-d-old capsules after pollination were first germinated on half-strength Murashige and Skoog (MS) basal medium supplemented with 1 mg L<sup>-1</sup> of naphthalene acetic acid (NAA) + 2 mg L<sup>-1</sup> of benzyl aminopurine (BAP). For multiple shoot induction, the axenic nodal segments excised from 120-d-old seedlings were cultured on the medium containing different concentrations and combinations of NAA and BAP. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L<sup>-1</sup> of indole-3 acetic acid (IAA) or indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L<sup>-1</sup> NAA and 2 mg L<sup>-1 </sup>BAP, while that for rooting with 1 mg L<sup>-1 </sup>IAA. The plantlets were hardened off and more than 90% of survived plants were released to the shade house. The plantlets flowered 1 yr after transplantation|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.08(4) [October 2009]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.