NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >


Title: Micropropagation of Dendrobium transparens L. from axenic pseudobulb segments
Authors: Sunitibala, H
Kishor, Rajkumar
Keywords: Axenic pseudobulb segment
benzyl aminopurine
Dendrobium transparens
in vitro
microshoot
Issue Date: Oct-2009
Publisher: CSIR
Abstract: An efficient protocol for micropropagation of Dendrobium transparens L. using the axenic pseudobulb segments, derived from in vitro germinated seedlings, was developed. The immature embryos from 120-d-old capsules after pollination were first germinated on half-strength Murashige and Skoog (MS) basal medium supplemented with 1 mg L-1 of naphthalene acetic acid (NAA) + 2 mg L-1 of benzyl aminopurine (BAP). For multiple shoot induction, the axenic nodal segments excised from 120-d-old seedlings were cultured on the medium containing different concentrations and combinations of NAA and BAP. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L-1 of indole-3 acetic acid (IAA) or indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L-1 NAA and 2 mg L-1 BAP, while that for rooting with 1 mg L-1 IAA. The plantlets were hardened off and more than 90% of survived plants were released to the shade house. The plantlets flowered 1 yr after transplantation
Page(s): 448-452
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

Files in This Item:

File Description SizeFormat
IJBT 8(4) 448-452.pdf395.72 kBAdobe PDFView/Open
 Current Page Visits: 772 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 603162 since 06-Feb-2009  Last updated on 21-Oct-2014Webmaster: nopr@niscair.res.in