Micropropagation of <i style="">Dendrobium transparens</i> L. from axenic pseudobulb segments

Abstract

An efficient protocol for micropropagation of <i style="">Dendrobium transparens</i> L. using the axenic pseudobulb segments, derived from <i style="">in vitro</i> germinated seedlings, was developed. The immature embryos from 120-d-old capsules after pollination were first germinated on half-strength Murashige and Skoog (MS) basal medium supplemented with 1 mg L^-1 of naphthalene acetic acid (NAA) + 2 mg L^-1 of benzyl aminopurine (BAP). For multiple shoot induction, the axenic nodal segments excised from 120-d-old seedlings were cultured on the medium containing different concentrations and combinations of NAA and BAP. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L^-1 of indole-3 acetic acid (IAA) or indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L^-1 NAA and 2 mg L^-1BAP, while that for rooting with 1 mg L^-1IAA. The plantlets were hardened off and more than 90% of survived plants were released to the shade house. The plantlets flowered 1 yr after transplantation