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Title: Induction of morphogenic callus and multiple shoot regeneration in Momordica cymbalaria Fenzl
Authors: Nikam, T D
Ghane, S G
Nehul, J N
Barmukh, R B
Keywords: Callus
in vitro propagation
Momordica cymbalaria
Issue Date: Oct-2009
Publisher: CSIR
Abstract: An in vitro propagation protocol for a wild vegetable and an ethnomedicinal plant, Momordica cymbalaria Fenzl., has been developed. The influences of 0.0-5.0 M 6-BA, 0.0-20.0 M Kn alone and in combination with 2.5- 5.0 M IAA, 2.5-5.0 M NAA and 2.5-5.0 M 2,4-D on in vitro multiple shoot production from node, internode and leaf explants was studied. The maximum number of indirect (callus interspersed) regeneration of multiple shoots (9.0±0.5 shoots per explant) was achieved from leaf explants on MS medium enriched with 2.5 M BA alone. Further, large-scale shoot formation (35±3.4 shoots per culture) was achieved by repeated subculturing of leaf-callus on shoot regeneration medium (MS+2.5 M BA). The capacity of large-scale shoot regeneration remained constant in the callus over a period of 2 years. The best root induction (100%) and survival (88%) was achieved on hormone free half strength MS medium. Addition of IAA or NAA in rooting medium induced callus formation in the shoots. Field established plants showed uniform growth and were morphologically identical to the parental stock.
Page(s): 442-447
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

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