Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6144
Title: Amyloglucosidase from <i>Rhizopus</i> mold and <img src='/image/spc_char/beta.gif'>-glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides
Authors: Sivakumar, Ramaiah
Divakar, Soundar
Keywords: Amyloglucosidase
carbohydrates
<img src='/image/spc_char/beta.gif'>-glucosidase
regioselectivity
riboflavin
riboflavinyl glycosides
Rhizopus
sweet almond
Issue Date: Oct-2009
Publisher: CSIR
Abstract: Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from <i>Rhizopus</i> mold and <img src='/image/spc_char/beta.gif'>-glucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, <i style="">p</i>H, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, D-mannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1<img src='/image/spc_char/alpha.gif'> and C1<img src='/image/spc_char/beta.gif'> glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-<i>O</i>-arylated products of D-glucose and maltose and C1-<i>O</i>-arylated product of sucrose. However, <img src='/image/spc_char/beta.gif'>-glucosidase gave C1<img src='/image/spc_char/beta.gif'>-glucoside, C1<img src='/image/spc_char/beta.gif'>-lactoside and C1<img src='/image/spc_char/alpha.gif'> and C1<img src='/image/spc_char/beta.gif'> glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC<sub>50</sub> values in the 0.8±0.04-2.08±0.1 m<i style="">M</i> range.
Description: 400-407
URI: http://hdl.handle.net/123456789/6144
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.08(4) [October 2009]

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