NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >

Title: Amyloglucosidase from Rhizopus mold and -glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides
Authors: Sivakumar, Ramaiah
Divakar, Soundar
Keywords: Amyloglucosidase
riboflavinyl glycosides
sweet almond
Issue Date: Oct-2009
Publisher: CSIR
Abstract: Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and -glucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, D-mannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1 and C1 glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-O-arylated products of D-glucose and maltose and C1-O-arylated product of sucrose. However, -glucosidase gave C1-glucoside, C1-lactoside and C1 and C1 glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC50 values in the 0.8±0.04-2.08±0.1 mM range.
Page(s): 400-407
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

Files in This Item:

File Description SizeFormat
IJBT 8(4) 400-407.pdf103.75 kBAdobe PDFView/Open
 Current Page Visits: 144 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 162946 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: