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Title: Amyloglucosidase from Rhizopus mold and -glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides
Authors: Sivakumar, Ramaiah
Divakar, Soundar
Keywords: Amyloglucosidase
carbohydrates
-glucosidase
regioselectivity
riboflavin
riboflavinyl glycosides
Rhizopus
sweet almond
Issue Date: Oct-2009
Publisher: CSIR
Abstract: Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and -glucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, D-mannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1 and C1 glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-O-arylated products of D-glucose and maltose and C1-O-arylated product of sucrose. However, -glucosidase gave C1-glucoside, C1-lactoside and C1 and C1 glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC50 values in the 0.8±0.04-2.08±0.1 mM range.
Page(s): 400-407
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

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