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|Title:||Amyloglucosidase from Rhizopus mold and -glucosidase from sweet almond catalysed syntheses of riboflavinyl glycosides|
|Keywords:||Amyloglucosidase;carbohydrates;-glucosidase;regioselectivity;riboflavin;riboflavinyl glycosides;Rhizopus;sweet almond|
|Abstract:||Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and -glucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, D-mannose, D-ribose, sucrose, maltose and lactose, both the enzymes gave yields in the range of 5-40%. Amyloglucosidase catalysis gave C1 and C1 glycosides of D-glucose, D-galactose, D-mannose, D-ribose, maltose, C6-O-arylated products of D-glucose and maltose and C1-O-arylated product of sucrose. However, -glucosidase gave C1-glucoside, C1-lactoside and C1 and C1 glycosides of D-galactose and D-mannose. Riboflavinyl glycosides showed angiotensin converting enzyme inhibitory activities, with IC50 values in the 0.8±0.04-2.08±0.1 mM range.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.08(4) [October 2009]|
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