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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >
| Title: | Development and molecular characterization of interspecific hybrids of Jatropha curcas × J. integerrima |
| Authors: | Dhillon, R S
Hooda, M S
Jattan, M
Chawla, V
Bhardwaj, M
Goyal, S C |
| Keywords: | Jatropha
interspecific hybrids
RAPD markers |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | Reciprocal crosses between J. curcas
and J. integerrima were attempted.
The interspecific hybrids were successful only when the former was used as seed
parent. The F1 hybrids exhibited a wide range of variation for
vegetative and reproductive traits suggesting considerable heterogeneity
between the parental plants. Some of the characters such as stem type,
branching habit, leaf size and shape of the hybrids resembled the female parent
while others like leaf pigmentation, fruit and seed size, flower colour,
resembled the male parent. The parents and their 23 hybrid plants were
subjected to random amplified polymorphic DNA (RAPD) analysis using 61 random
decamer primers. Out of 61 primers, the amplification was obtained in 51
primers producing 384 bands. The similarity value between the parents (41.4%)
was less than those between parents and hybrids. Cluster analysis based on
Jaccard’s similarity coefficient outgrouped the female parent from rest of the
genotypes at a similarity coefficient of 0.55. The 2-Dimensional and
3-Dimensional scaling by principal component analysis (PCA) grouped some
hybrids with male parent, some with female parent while others in between both
the parents |
| Page(s): | 384-390 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(4) [October 2009]
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