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IJBT Vol.08(4) [October 2009] >


Title: Molecular characterization of Pestalotiopsis spp. associated with tea (Camellia sinensis) in southern India using RAPD and ISSR markers
Authors: Joshi, Sarvottam D
Sanjay, R
Baby, U I
Mandal, A K A
Keywords: Camellia sinensis
Pestalotiopsis
RAPD
ISSR
phylogenetic
PCR
tea
Issue Date: Oct-2009
Publisher: CSIR
Abstract:  The genus Pestalotiopsis is second most important fungal pathogen causing grey blight disease in tea plant (Camellia sinensis). Due to grey blight disease, total tea crop loss is estimated to be 17% in southern India. In the present study, 42 Pestalotiopsis isolates were collected from five different regions in southern India. Among them, 22 isolates showed diverse morphological characters like colour, size and length of conidia and virulence. Genetic diversity was studied for these 22 isolates using two molecular marker systems (RAPD and ISSR). In RAPD, a total of 255 loci were generated and all were polymorphic in nature and the band size ranged from 0.2 to 3.0 kb. In ISSR, 194 amplified loci were observed and all were polymorphic as like RAPD and the band size ranged from 0.25 to 3.2 kb. Using Jaccard’s similarity coefficient matrix, highest similarity of 95.9% and 92.6% was observed between AP-8 and AP-9 isolates in both RAPD and ISSR markers, respectively. Lowest similarity was observed between AP-14 and NP-5 (8.8%) in RAPD matrix but in ISSR matrix lowest similarity (18.5%) was between AP-14 and EN-5. The UPGMA clustering of both methods was comparable. The results indicate that, within southern India, the diversity of Pestalotiopsis was high both morphologically and genetically
Page(s): 377-383
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

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