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IJBT Vol.08 [2009] >
IJBT Vol.08(4) [October 2009] >
| Title: | Genetic diversity and relationships among tea (Camellia sinensis) cultivars as revealed by RAPD and ISSR based fingerprinting |
| Authors: | Roy, S C
Chakraborty, B N |
| Keywords: | Dendrogram
genetic diversity
gene flow
RAPD-ISSR fingerprinting
tea |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: |
Genomic fingerprinting in 21 tea genotypes was carried out using 7 ISSR and
12 RAPD primers. Polymorphism was 88.54% in ISSR fingerprinting, but was 77.77%
in case of RAPD based fingerprinting. Dendrogram was constructed on the basis
of genetic similarity matrix using the UPGMA algorithm, which showed three
clusters (China type, Assam type and
Cambod type). The
genetic diversity over all groups (HT)
on an average was 0.38, diversity within populations (HS) was 0.27, and genetic differentiation (GST) between populations over
all loci was 0.25. The China variety had
shown the largest within-group diversity (HS
= 0.285-0.291), while the Cambod tea had the least diversity (HS = 0.193-0.207)
and moderate diversity existed in Assam tea (HS = 0.223-0.241). Interpopulation gene flow [Nm = 0.5(1 – GST)/GST] was 0.76; Nm < 1.0 shows the limited genetic exchange among populations. |
| Page(s): | 370-376 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.08(4) [October 2009]
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