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IJBT Vol.08(4) [October 2009] >


Title: Genetic diversity and relationships among tea (Camellia sinensis) cultivars as revealed by RAPD and ISSR based fingerprinting
Authors: Roy, S C
Chakraborty, B N
Keywords: Dendrogram
genetic diversity
gene flow
RAPD-ISSR fingerprinting
tea
Issue Date: Oct-2009
Publisher: CSIR
Abstract:  Genomic fingerprinting in 21 tea genotypes was carried out using 7 ISSR and 12 RAPD primers. Polymorphism was 88.54% in ISSR fingerprinting, but was 77.77% in case of RAPD based fingerprinting. Dendrogram was constructed on the basis of genetic similarity matrix using the UPGMA algorithm, which showed three clusters (China type, Assam type and Cambod type). The genetic diversity over all groups (HT) on an average was 0.38, diversity within populations (HS) was 0.27, and genetic differentiation (GST) between populations over all loci was 0.25. The China variety had shown the largest within-group diversity (HS = 0.285-0.291), while the Cambod tea had the least diversity (HS = 0.193-0.207) and moderate diversity existed in Assam tea (HS = 0.223-0.241). Interpopulation gene flow [Nm = 0.5(1 – GST)/GST] was 0.76; Nm < 1.0 shows the limited genetic exchange among populations.
Page(s): 370-376
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.08(4) [October 2009]

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