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JSIR Vol.68(10) [October 2009] >


Title: Characterization of a partially purified bacteriocin of Bacillus sp MTCC 43 isolated from Rhizosphere of radish (Raphanus sativus) & its application as a potential food biopreservative
Authors: Sharma, N
Kapoor, G
Gautam, N
Neopaney, B
Keywords: Bacteriocin
Bacillus sp. MTCC 43
Food pathogens
Raphanus sativus
Issue Date: Oct-2009
Publisher: CSIR
Abstract: This study presents bacteriocin, produced from Bacillus sp. MTCC 43 isolated from rhizosphere of root crop radish, Raphanus sativus, as inhibitor against serious food pathogens (Staphylococcus aureus and Aeromonas hydrophila). Bacteriocin of Bacillus sp. MTCC 43, partially purified by salt saturation method, exhibits very high activity (2.66 x 105 AU/ml). Viability of pathogens decreased drastically (up to 70%) within first 10 h of adding bacteriocin. Bacteriocin showed high thermostability (up to 100°C) for 10 min, expressed wide pH tolerance (4.0-10.0), and found sensitive to proteolytic enzyme trypsin.
Page(s): 881-886
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.68(10) [October 2009]

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