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Title: Characterization of a partially purified bacteriocin of <i>Bacillus sp </i>MTCC 43 isolated from Rhizosphere of radish <i>(Raphanus sativus) </i>& its application as a potential food biopreservative
Authors: Sharma, N
Kapoor, G
Gautam, N
Neopaney, B
Keywords: Bacteriocin
<i>Bacillus </i>sp. MTCC 43
Food pathogens
<i>Raphanus sativus</i>
Issue Date: Oct-2009
Publisher: CSIR
Abstract: This study presents bacteriocin, produced from <i>Bacillus </i>sp. MTCC 43 isolated from rhizosphere of root crop radish, <i>Raphanus sativus, </i>as inhibitor against serious food pathogens (<i>Staphylococcus aureus </i>and <i>Aeromonas hydrophila</i>). Bacteriocin of <i>Bacillus </i>sp. MTCC 43, partially purified by salt saturation method, exhibits very high activity (2.66 x 10<sup>5</sup> AU/ml). Viability of pathogens decreased drastically (up to 70%) within first 10 h of adding bacteriocin. Bacteriocin showed high thermostability (up to 100°C) for 10 min, expressed wide pH tolerance (4.0-10.0), and found sensitive to proteolytic enzyme trypsin.
Description: 881-886
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.68(10) [October 2009]

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