Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/6092
Title: Hypersensitive response of <i style="">Sesamum prostratum</i> Retz.<i style=""> </i>elicitated by <i style="">Fusarium oxysporum f. sesame</i> (Schelt) Jacz Butler.
Authors: Rajab, Reeja
Rajan, S Sajitha
Satheesh, L Shilpa
Harish, S R
Sunukumar, S S
Sandeep, B S
Mohan, T C Kishor
Murugan, K
Keywords: Antioxidant enzymes
Callus
<i style="">Fusarium oxysporum f. sesame</i>
Hypersensitive response
Oxidative burst
Reactive oxygen species
<i style="">Sesamum</i> <i>prostratum</i>
Issue Date: Oct-2009
Publisher: CSIR
Abstract: Aim of this study was to investigate the intensity and timing of the ROS formation, lipid peroxidation and expression of antioxidant enzymes as initial responses of calli of <i style="">Sesamum prostratum </i>(SP) against <i style="">Fusarium oxysporum</i> <i>f. sesame</i> crude toxin metabolite of varying concentrations. 2,4 dichlorophenoxy acetic acid (2,4-D) / coconut milk combinations were found to be more efficient among different hormonal regimes (2,4 -D, 2,4-D/casein hydrosylate and 2,4-D/ coconut milk). The concentration of hydrogen peroxide and lipid peroxidation were higher (13.2 and 5.7-folds, respectively) after 6 h in the treated callus confirmed the oxidative stress. An increase in total phenolics was also detected in inoculated callus. Increased activity of antioxidative enzymes viz., NADPH oxidase and superoxide dismutase (SOD) corroborate with the high level of ROSs, such as O<sub>2</sub><sup>.- </sup>and H<sub>2</sub>O<sub>2</sub>. The poor activity of catalase confirmed the oxidative burst in the callus leading to necrosis. Activity of peroxidase was at par with total phenolics. Similarly, phenylalanine ammonia lyase (PAL) also showed high activity revealing the active phase in the synthesis of secondary metabolites in the plant. The oxidative burst generated in the interaction between <i style="">Sesamum</i> and <i style="">F. oxysporum</i> <i>f. sesame</i> toxin might be the first line of defense by the host mounted against the invading necrotrophic pathogen. The results suggested that the rapid production of reactive oxygen species in the callus in response to fungal toxin had been proposed to orchestrate the establishment of different defensive barriers against the pathogens.<i style=""></i>
Description: 834-838
URI: http://hdl.handle.net/123456789/6092
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.47(10) [October 2009]

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