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Title: Kinetic characteristics of brush border sucrase activation by Na+ ions in mice intestine
Authors: Gupta, Shiffalli
Mahmood, Safrun
Mahmood, Akhtar
Keywords: Brush border sucrase
Mice intestine
Na+ activation kinetics
pH dependent effects
Issue Date: Oct-2009
Publisher: CSIR
Abstract:  The kinetics of Na+ activation of brush border sucrase (sucrose D-glucosidase E.C. 3.2.1.48) has been studied in mice intestine. At pH 5.0, 50 mM Na+ ions stimulated sucrase activity by 84%. At pH 7.2, enzyme stimulation was reduced to 16%, whereas, at pH 8.5, 10-100 mM Na+ ions produced 18-45% inhibition of enzyme activity. Kinetic studies revealed that at pH 5.0, the enzyme activation by Na+ ions was V-type, which changed to K-type at pH 7.2, whereas at alkaline pH (8.5), Na+ ions inhibited the enzyme activity non-competitively. Using the non-compulsory model of Na+ ion stimulation of brush border sucrase [Mahmood & Alvarado, Arch Bioch Biophys, 168 (1975) 585] various kinetic constants involved in activation of sucrase by Na+ ions were determined. It is apparent that Na+ stimulation of brush border sucrase is pH dependent, which is similar to that described for rat, rabbit and other mammalian species and conform to identical mechanisms, at least with reference to the affinity type effects, as observed in mice intestine.
Page(s): 811-815
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.47(10) [October 2009]

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