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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(10) [October 2009] >
| Title: | Cloning, expression and characterisation of a promising mosquitocidal gene |
| Authors: | Zhang, Lingling
Huang, Enjiong
Gelbič, Ivan
Guan, Chunyu
Guan, Yi
Guan, Xiong |
| Keywords: | Bacillus thuringiensi
cyt1Aa6
Expression
Molecular cloning
Purification |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | A new mosquitocidal gene, cyt1Aa6, was isolated and cloned from the novel Bacillus thuringiensis strain LLP29, previously isolated from the
phylloplane of Magnolia denudata.
Nucleotide sequence analysis of cyt1Aa6
indicated that the open reading frame consisted of 750 base pairs, encoding 249
amino acid sequences with a calculated molecular weight of 27.3681 kDa and a PI
value of 4.77. An homological comparison revealed that the cyt1Aa6 amino acid sequence was 99% identical with those of known
Cyt1Aa proteins. In addition, the cyt1Aa6
gene was successfully expressed in Escherichia
coli BL21. Bioassays on Aedes
albopictus showed that Bt LLP29 and the expressed BL21 were both toxic to
3rd-instar mosquito larvae. The isolation of cyt1Aa6 provides new opportunities for
selecting new strains and to obtain novel B. thuringiensis products based on its toxins. |
| Page(s): | 799-803 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.47(10) [October 2009]
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