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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.47 [2009] >
IJEB Vol.47(10) [October 2009] >
| Title: | Comparative genomic hybridization array study and its utility in detection of constitutional and acquired anomalies |
| Authors: | Andrieux, Joris
Sheth, Frenny |
| Keywords: | Acquired anomalies
Array-CGH
Constitutional disorders
Molecular karyotyping |
| Issue Date: | Oct-2009 |
| Publisher: | CSIR |
| Abstract: | The last decade has witnessed an upsurge in the knowledge of
cytogenetic disorders and putting the old technology in a new basket with
molecular genetics. As conventional cytogenetic can detect the genetic
alteration of 10-15 Mb, many of the micro-deletions and micro-duplications are
missed. However, with the advent of technology of fluorescence in situ hybridization (FISH), the
resolution of genetic aberrations can reach to 3-5 Mb, nonetheless the
anomalies smaller than the above, need further precision which has been achieved
using comparative genomic hybridization array (CGH-array). Introduction of
array-CGH has brought higher sensitivity with automated DNA fragment analyzer
and DNA chip for submicroscopic chromosomal anomalies that are missed till date
in many of the acquired and constitutional genetic disorders. The resolution of
the technology varies from several Kb to 1 Mb depending upon the type of array
selected. With the recent improvement in the array-CGH technology, a link
between cytogenetic and molecular biology has been established without replacing
conventional cytogenetic technique. The wider
accessibility of the technology shall certainly provide a clue to the many
unidentified/unexplained genetic disorders which shall prove to be a boon to
the clinicians. |
| Page(s): | 779-791 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.47(10) [October 2009]
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