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|Title:||Laboratory scale bioremediation of diesel hydrocarbon in soil by indigenous bacterial consortium|
Rehman, Meenal Budholia
|Abstract:||<i style="">In vitro</i> experiment was performed by taking petrol pump soils and diesel in flasks with the micronutrients and macronutrients supplements. Cemented bioreactors having sterilized soil and diesel was used for <i style="">in vivo</i> analysis of diesel hydrocarbon degradation. There were two sets of experiments, first having three bioreactors (1) inoculated by <i>Kl</i>. <i style="">pneumoniae</i> subsp. <i style="">aerogenes</i> with soil and diesel; (2) with addition of NH<sub>4</sub>NO<sub>3</sub>; and (3) served as control. In second set, one bioreactor was inoculated by bacterial consortium containing <i style="">Moraxella</i> <i style="">saccharolytica, Alteromonas putrefaciens</i>, <i>Kl</i>. <i style="">pneumoniae</i> subsp. <i style="">aerogenes</i> and <i style="">Pseudomonas fragi</i> along with soil and diesel. The remaining two bioreactors (having NH<sub>4</sub>NO<sub>3</sub> and control) were similar to the first set. The experiments were incubated for 30 days. Ability of bacterial inoculum to degrade diesel was analyzed through GC-MS. Smaller chain compounds were obtained after experimental period of 30 days. Rate of diesel degradation was better with the present bacterial consortium than individual bacteria. Present bacterial consortium can be a better choice for faster and complete remediation of contaminated hydrocarbon soils.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.47(09) [September 2009]|
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