NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(4) [October 2004] >

Title: Detection and molecular characterisation of waterborne pathogenic Escherichia coli using multiplex PCR
Authors: Thomas, Sabu
Krishnan, K Hari
Narayanan, Manoj
Nair, Sandhya C
Keywords: antibiogram
drinking water
plasmid profile
SLT producing E. coli
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeInt. Cl.7 C 12 N 15/10; C 12 P 29/00, 35/00
Abstract: Specific culture methods were adopted for the isolation of lactose fermenting bacteria from various water sources. MPN study revealed that the drinking water sources in Kottarakara area of Kollam District, Kerala were polluted with faecal coliforms. A multiplex PCR (mPCR) protocol was used for the identification of Escherichia coli strains. In mPCR, only six isolates gave a 224/227 bp fragment of the SLT I/SLT II genes of E. coli. Antibiogram studies showed that all E. coli isolates were resistant to many of the drugs used in the study. However, Carbenicillin, Chloramphenicol and Tetracyclin were sensitive to all the isolates.
Page(s): 603-605
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(4) [October 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(4) 603-605.pdf157.63 kBAdobe PDFView/Open
 Current Page Visits: 816 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 170469 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: