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Title: Detection and molecular characterisation of waterborne pathogenic Escherichia coli using multiplex PCR
Authors: Thomas, Sabu
Krishnan, K Hari
Narayanan, Manoj
Nair, Sandhya C
Keywords: antibiogram;bacteriology;drinking water;plasmid profile;SLT producing E. coli
Issue Date: Oct-2004
Publisher: CSIR
IPC Code: Int. Cl.7 C 12 N 15/10; C 12 P 29/00, 35/00
Abstract: Specific culture methods were adopted for the isolation of lactose fermenting bacteria from various water sources. MPN study revealed that the drinking water sources in Kottarakara area of Kollam District, Kerala were polluted with faecal coliforms. A multiplex PCR (mPCR) protocol was used for the identification of Escherichia coli strains. In mPCR, only six isolates gave a 224/227 bp fragment of the SLT I/SLT II genes of E. coli. Antibiogram studies showed that all E. coli isolates were resistant to many of the drugs used in the study. However, Carbenicillin, Chloramphenicol and Tetracyclin were sensitive to all the isolates.
Page(s): 603-605
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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