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IJCT Vol.16(4) [July 2009] >


Title: Enzymatic synthesis of fructose ester from mango kernel fat
Authors: Dandekar, P P
Patravale, V B
Keywords: Biosurfactant
Fructose ester
Mangifera indica
Mango kernel fat (MKF)
Candida rugosa
Issue Date: Jul-2009
Publisher: CSIR
Abstract: Fructose ester as a biosurfactant was successfully synthesized from the fatty acid fraction (olein fraction) of Mango Kernel Fat (MKF). The synthesis was carried out by reaction of sugar and fatty acid fraction in presence of the enzyme lipase from Candida rugosa which acted as a biocatalyst. Phosphate buffer (pH 7.0) was used as the reaction medium to realise maximum enzymatic action. The product was separated from the reaction medium by liquid- liquid extraction. Maximum conversion (36.52 %) was achieved at fructose to olein fraction molar ratio of 1 : 10 (mol/L), with the lipase concentration of 4g/L, at a temperature of 30oC at the end of 3 days. The presence of an ester band in the synthesized ester was confirmed by Fourier Transform Infrared (FTIR) analysis. Identity of the product was further confirmed by NMR studies and a colour identity test for the ester group.
Page(s): 317-321
ISSN: 0975-0991 (Online); 0971-457X (Print)
Source:IJCT Vol.16(4) [July 2009]

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