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Title: Factors influencing initiation of embryogenic cultures in Pinus kesiya Royle ex Gord.
Authors: Deb, Chitta Ranjan
Tandon, Pramod
Keywords: activated charcoal treatment
MS medium
Pinus kesiya
seed imbibition period
somatic embryogenesis
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 4/00, 7/00
Abstract:  Seeds, 5-6 week old, secondary needles and apical domes of Pinus kesiya were collected during January-February, March-June and May-July, respectively. The explants were primed before initiation of culture. The embryogenic cultures were obtained on mMS medium from zygotic embryos (79.6%), dissected out from imbibed seeds (for 24 hrs at 4°C), on MS medium from secondary needles (88.6%) and on ½DCR medium from 0.2-0.5 mm thick pre-cultured (on ½DCR medium, containing 2% sucrose, 0.4% activated charcoal, at 4°C for 72 h) apical dome sections (92.6%). All media were supplemented with 2% sucrose, 1000 mg l-l casein-hydrolysate, 1000 mg l-l myo-inositol and 500 mg l-l l-glutamine, adjusted to pH 5.5. For initiation of embryogenic cultures, 5 mg l-l each of 2,4-D and NAA along with 2.5 mg l-l BAP were incorporated to media for both zygotic embryos and apical dome sections, while 3.0 mg l-l of 2,4-D and NAA each along with 1.0 mg l-l BAP were incorporated for secondary needles. The proembryonal masses and proembryos were developed from embryogenic cultures of zygotic embryos and secondary needles, and cold treated (at 4oC for 24 h) cultures of apical dome sections by culturing them on their basal media containing 1/10th level of growth regulators followed by growth regulator free media. Further, the cotyledonary embryos were formed on their respective basal media containing 4% sucrose and 4 mg l-l ABA.
Page(s): 589-593
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(4) [October 2004]

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