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Title: Factors influencing initiation of embryogenic cultures in <i style="">Pinus kesiya</i> Royle ex Gord.
Authors: Deb, Chitta Ranjan
Tandon, Pramod
Keywords: activated charcoal treatment
MS medium
<i style="">Pinus kesiya</i>
seed imbibition period
somatic embryogenesis
Issue Date: Oct-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 7/00
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> Seeds, 5-6 week old, secondary needles and apical domes of <i style="">Pinus kesiya</i> were collected during January-February, March-June and May-July, respectively. The explants were primed before initiation of culture. The embryogenic cultures were obtained on mMS medium from zygotic embryos (79.6%), dissected out from imbibed seeds (for 24 hrs at 4°C), on MS medium from secondary needles (88.6%) and on ½DCR medium from 0.2-0.5 mm thick pre-cultured (on ½DCR medium, containing 2% sucrose, 0.4% activated charcoal, at 4°C for 72 h) apical dome sections (92.6%). All media were supplemented with 2% sucrose, 1000 mg l<sup>-l</sup> casein-hydrolysate, 1000 mg l<sup>-l</sup> myo-inositol and 500 mg l<sup>-l</sup> l-glutamine, adjusted to <i style="">p</i>H 5.5. For initiation of embryogenic cultures, 5 mg l<sup>-l</sup> each of 2,4-D and NAA along with 2.5 mg l<sup>-l</sup> BAP were incorporated to media for both zygotic embryos and apical dome sections, while 3.0 mg l<sup>-l</sup> of 2,4-D and NAA each along with 1.0 mg l<sup>-l</sup> BAP were incorporated for secondary needles. The proembryonal masses and proembryos were developed from embryogenic cultures of zygotic embryos and secondary needles, and cold treated (at 4<sup>o</sup>C for 24 h) cultures of apical dome sections by culturing them on their basal media containing 1/10<sup>th</sup> level of growth regulators followed by growth regulator free media. Further, the cotyledonary embryos were formed on their respective basal media containing 4% sucrose and 4 mg l<sup>-l</sup> ABA. </smarttagtype></smarttagtype>
Description: 589-593
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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