|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(4) [October 2004] >
| Title: | Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants |
| Authors: | Ghanti, Kiran
Kaviraj, C P
Venugopal, R B
Jabeen, F T Z
Rao, Srinath |
| Keywords: | BAP
GA3
IBA
Mentha piperita
MS medium
NAA
zeatin |
| Issue Date: | Oct-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | A high frequency and rapid regeneration protocol was developed from shoot tip and nodal explants of Mentha piperita L. on Murashige and Skoog’s (MS) medium supplemented with either 6-benzyl amino purine (BAP; 1 mg/l)) or zeatin (0.25 mg/l). The highest number of shoots (49.8) was obtained on medium containing BAP. The regenerated dwarf shoots were further elongated on MS medium supplemented with gibberellic acid (GA3; 1 mg/l). In vitro shoots were then excised from shoot clumps and transferred to rooting medium containing naphthalene acetic acid (NAA; 1 mg/l). The rooted plantlets were hardened on MS basal liquid medium and subsequently in polycups containing sterile soil and vermiculite (1:1). Plantlets, thus, developed were successfully established and finally transferred to a greenhouse. The plantlets showed high survival rate (90%) in the soil. |
| Page(s): | 594-598 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(4) [October 2004]
|
|
