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Title: Rapid regeneration of Mentha piperita L. from shoot tip and nodal explants
Authors: Ghanti, Kiran
Kaviraj, C P
Venugopal, R B
Jabeen, F T Z
Rao, Srinath
Keywords: BAP;GA3;IBA;Mentha piperita;MS medium;NAA;zeatin
Issue Date: Oct-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 H 4/00, 5/00
Abstract: A high frequency and rapid regeneration protocol was developed from shoot tip and nodal explants of Mentha piperita L. on Murashige and Skoog’s (MS) medium supplemented with either 6-benzyl amino purine (BAP; 1 mg/l)) or zeatin (0.25 mg/l). The highest number of shoots (49.8) was obtained on medium containing BAP. The regenerated dwarf shoots were further elongated on MS medium supplemented with gibberellic acid (GA3; 1 mg/l). In vitro shoots were then excised from shoot clumps and transferred to rooting medium containing naphthalene acetic acid (NAA; 1 mg/l). The rooted plantlets were hardened on MS basal liquid medium and subsequently in polycups containing sterile soil and vermiculite (1:1). Plantlets, thus, developed were successfully established and finally transferred to a greenhouse. The plantlets showed high survival rate (90%) in the soil.
Page(s): 594-598
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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