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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(4) [October 2004] >
| Title: | Rapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants |
| Authors: | Tyagi, Purnima
Kothari, S L |
| Keywords: | Gerbera jamesonii
capitulum
leaf
in vitro regeneration |
| Issue Date: | Oct-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | Rapid in vitro multiplication of shoots has been described using capitular sections and leaf explants in Gerbera jamesonii. Three types of basal media regimes were used including MS and two of its modified forms¾MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular sections and Kn (4 mg/l) and IAA (0.1 mg/l) from leaves (5-8 shoot buds). MSI medium with 2 mg/l Kn+0.5 mg/l PAA was used for rapid multiplication of organogenic callus; 20-25 shoot buds developed from this callus. Rooting of in vitro shoots was achieved on MS medium with 0.5 mg/l IAA. Plantlets were transferred to pots where they matured and flowered. |
| Page(s): | 584-588 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(4) [October 2004]
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