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Title: Rapid <i style="">in vitro</i> regeneration of <i style="">Gerbera jamesonii</i> (H. Bolus ex Hook. f.) from different explants
Authors: Tyagi, Purnima
Kothari, S L
Keywords: <i style="">Gerbera jamesonii</i>
<i style="">in vitro</i> regeneration
Issue Date: Oct-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 5/00
Abstract: Rapid <i style="">in vitro </i>multiplication of shoots has been described using capitular sections and leaf explants in <i style="">Gerbera jamesonii</i>. Three types of basal media regimes were used including MS and two of its modified formsĀ¾MSI and MSA. Shoot induction was achieved on all three regimes but MSI medium supplemented with Kn (4 mg/l) and IAA (0.5 mg/l) was ideal for shoot bud initiation (8-11) from capitular sections and Kn (4 mg/l) and IAA (0.1 mg/l) from leaves (5-8 shoot buds). MSI medium with 2 mg/l Kn+0.5 mg/l PAA was used for rapid multiplication of organogenic callus; 20-25 shoot buds developed from this callus. Rooting of <i style="">in vitro</i> shoots was achieved on MS medium with 0.5 mg/l IAA. Plantlets were transferred to pots where they matured and flowered.
Description: 584-588
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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