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Title: Production of extracellular pectinolytic, cellulolytic and xylanoytic enzymes by thermophilic mould <i style="">Sporotrichum thermophile</i> Apinis in solid state fermentation
Authors: Kaur, Guneet
Satyanarayana, T
Keywords: citrus pectin
<i style="">Sporotrichum thermophile</i>
wheat bran
Issue Date: Oct-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 N 63/00
Abstract: Among four thermophilic moulds, <i style="">Sporotrichum thermophile</i> produced high titres of xylanases, pectinases and cellulases after 4 days of incubation in solid-state fermentation (SSF). Of the 27 different combinations of agro-residues tried, wheat bran (WB) and citrus pectin (CP) in 1:1 ratio supported a very high production of enzymes. When the mixed substrate at <i style="">p</i>H 7.0 was moistened with tap water (1:2.5 ratio) to a<sub>w</sub> of 0.95 and inoculated with 60 <img src='/image/spc_char/cross.gif'> 10<sup>7</sup> conidiospores (from 5 day-old culture) 10<sup>-1</sup> g of substrate, <i style="">S</i>.<i style=""> thermophile </i>secreted maximum enzyme titres (xylanase 1900, pectinase 250 and cellulase 42 Ug<sup>-1</sup> dry mouldy bran in 4 days at 45°C. The mixture of enzymes has been found useful in the treatment of fruit pulps for enhanced juice recovery.
Description: 552-557
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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