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Title: Production of extracellular pectinolytic, cellulolytic and xylanoytic enzymes by thermophilic mould Sporotrichum thermophile Apinis in solid state fermentation
Authors: Kaur, Guneet
Satyanarayana, T
Keywords: citrus pectin;cellulase;pectinase;Sporotrichum thermophile;SSF;thermostable;wheat bran;xylanase
Issue Date: Oct-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 N 63/00
Abstract: Among four thermophilic moulds, Sporotrichum thermophile produced high titres of xylanases, pectinases and cellulases after 4 days of incubation in solid-state fermentation (SSF). Of the 27 different combinations of agro-residues tried, wheat bran (WB) and citrus pectin (CP) in 1:1 ratio supported a very high production of enzymes. When the mixed substrate at pH 7.0 was moistened with tap water (1:2.5 ratio) to aw of 0.95 and inoculated with 60 107 conidiospores (from 5 day-old culture) 10-1 g of substrate, S. thermophile secreted maximum enzyme titres (xylanase 1900, pectinase 250 and cellulase 42 Ug-1 dry mouldy bran in 4 days at 45°C. The mixture of enzymes has been found useful in the treatment of fruit pulps for enhanced juice recovery.
Page(s): 552-557
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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