NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(4) [October 2004] >

Title: Rapid diagnosis of sugarcane red rot by Dot-immunobinding assay (DIBA) technique
Authors: Hiremath, Lingayya
Naik, G R
Keywords: Colletotrichum falcatum
red rot
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeInt Cl.7 G 01 N 33/53
Abstract: A high sugar and early maturing, sugarcane var. CoC.671 has been found susceptible to red rot disease caused by Colletotrichum falcatum Went. However, during early stages in the field, diagnosis of the disease is very difficult. Therefore, a protocol for rapid diagnosis of sugarcane red rot infection by using DIBA technique is described in the present paper. Antigenic proteins of C. falcatum were SDS-PAGE separated on 12% gel, whereas DIBA was performed on nitrocellulose membrane. A series of dilutions of infected test samples depicted dark blue precipitate on the nitrocellulose membrane due to the antigen and antibody reaction. This indicated the presence of red rot antigen in the test sample and hence the disease. The DIBA described in this study is simple, rapid and specific for laboratory diagnosis of sugar cane (var. CoC.671) red rot infection in the planting material at an early growth stage.
Page(s): 542-545
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(4) [October 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(4) 542-545.pdf209.88 kBAdobe PDFView/Open
 Current Page Visits: 1186 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 162773 since 01-Sep-2015  Last updated on 21-Jun-2016Webmaster: