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Title: Isolation and characterization of mRNAs differentially expressed during ripening of mango fruits
Authors: Saiprasad, G V S
Anand, Lalitha
Ravishankar, K V
Mythili, J B
Nagesh, M
Joshi, R
Keywords: CCR-4;PRL-1;transcription factor;rRNA;RT-PCR
Issue Date: Oct-2004
Publisher: CSIR
IPC Code: Int. Cl7 C 12 N 15/10
Abstract: Mango, an important climacteric fruit crop with low shelf life, requires technologies to increase shelf life to reduce post harvest losses. During ripening of mango, the authors isolated five ripening related cDNAs from two mango varieties, Alphonso and Totapuri, using RT-PCR technique. The predicted polypeptides of five of these clones exhibit similarity to database protein sequences of PRL-1 protein, transcription initiation factor, CCR-4 protein, 18S ribosomal RNA gene and 23S ribosomal RNA gene. None of these proteins appear to be directly related to events generally associated with ripening such as cell wall metabolism or the accumulation of sugars and pigments or ethylene biosynthetic pathway. They are the regulatory elements/signals known to be involved during fruit ripening and may, therefore, be involved in regulating the expression of other genes directly associated with fruit ripening. The probable role of these proteins in mango fruit ripening needs to be elucidated further.
Page(s): 533-537
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(4) [October 2004]

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