NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(4) [October 2004] >

Title: Identification of microsatellite markers for differentiating some Basmati and non-Basmati rice varieties
Authors: Pal, Surender
Jain, Sunita
Saini, Navinder
Jain, Rajinder K
Keywords: Oryza sativa
genetic diversity
microsatellite markers
varietal differentiation
Issue Date: Oct-2004
Publisher: CSIR
IPC CodeInt. Cl.7 C 12 N 15/10
Abstract: Microsatellite marker (SSR) analysis was used to differentiate premium traditional Basmati rice varieties from other cheaper cross-bred Basmati/long-grain rice varieties and monitor the cases of adulteration in milled rice samples. Thirteen rice cultivars (4 commercial traditional Basmati, 6 cross-bred Basmati and 3 non-Basmati varieties) were evaluated for allelic diversity using 35 SSR markers. A total of 123 alleles (79-345 bp) were detected; 25 of these were present in Basmati rice varieties only. Polymorphism information content (PIC) value, which is indicative of level of polymorphism, varied from 0.0 (RM167) to 0.858 (RM252), with an average value of 0.447. SSR analysis generated polymorphism sufficient to differentiate all the 13 rice genotypes. Of the 35 markers, 16 showed amplification of a different allele in one or more of the traditional/cross-bred Basmati rice varieties than in IR36 (indica) and Azucena (japonica). Some SSRs (RM60, RM84, RM252, RM171, and RM257) were found unique among the closely related traditional Basmati rice varieties. Traditional Basmati rice varieties could be differentiated from one or more of the cross-bred Basmati rice varieties by allelic polymorphism at 27 of the 35 SSR loci; the most useful markers being RM171, RM1, RM44, RM110, RM229, RM234, RM242, and RM255. Rice varieties were clustered in three groups (indica, japonica, Basmati groups), which correspond well to their known pedigree data. This paper provides effective means to the Basmati traders for varietal differentiation and monitoring adulteration cases using milled rice samples.
Page(s): 519-526
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(4) [October 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(4) 519-526.pdf83.01 kBAdobe PDFView/Open
 Current Page Visits: 1110 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171090 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: