Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5886
Title: Plant regeneration from callus cultures in <i style="">Suaeda nudiflora </i>(Wild.) Moq.
Authors: Singh, Aneesha
Chikara, J
Pandya, J B
Keywords: <i style="">Suaeda nudiflora</i>
callus culture
organogenesis
benzylaminopurine
root primordial
hardened plantlets
Issue Date: Jul-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 5/00
Abstract: <i style="">Suaeda nudiflora </i>plants, showing relatively high salt tolerance, were used for micropropagation and generation of plantlets from callus cultures. Induction of somaclonal variability in the existing germplasm was another aim. Callus was initiated from young stem on MS medium supplemented with different concentrations of BAP and 2,4-D. Organogenesis was achieved by transferring callus on MS medium supplemented with BAP, KN and adenine. Well-grown shoots rooted easily on MS-half supplemented with IBA, IPA and NAA and 80-90% rooting was achieved. Plantlets got hardened by keeping them in hardening unit for few days. Hardened plants established very well in the nursery. Though originated from callus, these plants did not show any morphological variations and were similar to their parent donor plant.
Description: 454-456
URI: http://hdl.handle.net/123456789/5886
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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