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Title: Direct organogenesis in <i style="">Datura metel </i>L. from <i style="">in vitro</i> and <i style="">in vivo</i> nodal explants
Authors: Muthukumar, B
Arockiasamy, D I
Natarajan, E
Keywords: <i style="">Datura metel</i>
i<i style="">n vitro</i> regeneration
nodal explants
Issue Date: Jul-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 5/00
Abstract: <i style="">In vitro</i> plant regeneration was achieved in <i style="">Datura metel</i> from nodal explants collected from both <i style="">in vitro </i>germinated seedlings and field grown plants (<i style="">in vivo</i>). The explants were cultured on MS medium with BAP (0.5-3.0 mg/l) and NAA (0.5 mg/l). The nodal explants isolated from <i style="">in vivo </i>source exhibited a greater number of healthy multiple shoots than <i style="">in vitro</i>. BAP at 3 mg/l with NAA at 0.5 mg/l was found to be optimal for regeneration of shootlets.
Description: 449-451
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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