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Title: Direct organogenesis in Datura metel L. from in vitro and in vivo nodal explants
Authors: Muthukumar, B
Arockiasamy, D I
Natarajan, E
Keywords: Datura metel
medicinal
in vitro regeneration
nodal explants
BAP
organogenesis
Issue Date: Jul-2004
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 4/00, 5/00
Abstract: In vitro plant regeneration was achieved in Datura metel from nodal explants collected from both in vitro germinated seedlings and field grown plants (in vivo). The explants were cultured on MS medium with BAP (0.5-3.0 mg/l) and NAA (0.5 mg/l). The nodal explants isolated from in vivo source exhibited a greater number of healthy multiple shoots than in vitro. BAP at 3 mg/l with NAA at 0.5 mg/l was found to be optimal for regeneration of shootlets.
Page(s): 449-451
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(3) [July 2004]

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