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Title: Establishment of an embryogenic suspension culture of <i style="">Pinus kesiya</i> (Khasi pine) from various explants
Authors: Deb, Chitta Ranjan
Tandon, Pramod
Keywords: activated charcoal pre-treatment
embryogenic suspension culture
p<i style="">inus kesiya</i>
somatic embryogenesis
Issue Date: Jul-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 7/00
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"> The embryogenic cultures were obtained from mature zygotic embryos (79.6%) and apical dome sections (92.6%) (pretreated with 0.4% activated charcoal at 4<sup>o</sup>C for 24 hrs) on mMS and ½DCR media, respectively containing 5 mg l<sup>-1</sup> 2,4-D, NAA in combination along with 1 mg l<sup>-1</sup> BAP, while, from secondary needles (88.6%) it was on MS medium containing 3 mg l<sup>-1</sup> each of 2,4-D and NAA in conjunction with 1 mg l<sup>-1</sup> BAP. Using 150-200 mg embryogenic tissues per 15 ml medium at 120 rpm raised embryogenic suspension cultures. But the embryogenic cultures from apical dome sections were cold treated at 4<sup>o</sup>C for 24 hrs before transferring to suspension culture. The cultures were subcultured at 6-7 days interval and were diluted at 1:4 ratio. The embryonal suspensor masses developed from the resulting elongated single cells within 3-4 passages in respective basal medium with 1/10<sup>th</sup> growth regulators of initiation medium. The proembryonal head formed in growth regulators free medium at 100 rpm. The embryonal head formed in basal medium containing 4% sucrose and 4 mg l <sup>-1</sup> ABA in combination. </smarttagtype></smarttagtype>
Description: 445-448
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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