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Title: Establishment of an embryogenic suspension culture of Pinus kesiya (Khasi pine) from various explants
Authors: Deb, Chitta Ranjan
Tandon, Pramod
Keywords: activated charcoal pre-treatment;cold-treatment;embryogenic suspension culture;pinus kesiya;somatic embryogenesis
Issue Date: Jul-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 H 4/00, 7/00
Abstract:  The embryogenic cultures were obtained from mature zygotic embryos (79.6%) and apical dome sections (92.6%) (pretreated with 0.4% activated charcoal at 4oC for 24 hrs) on mMS and ½DCR media, respectively containing 5 mg l-1 2,4-D, NAA in combination along with 1 mg l-1 BAP, while, from secondary needles (88.6%) it was on MS medium containing 3 mg l-1 each of 2,4-D and NAA in conjunction with 1 mg l-1 BAP. Using 150-200 mg embryogenic tissues per 15 ml medium at 120 rpm raised embryogenic suspension cultures. But the embryogenic cultures from apical dome sections were cold treated at 4oC for 24 hrs before transferring to suspension culture. The cultures were subcultured at 6-7 days interval and were diluted at 1:4 ratio. The embryonal suspensor masses developed from the resulting elongated single cells within 3-4 passages in respective basal medium with 1/10th growth regulators of initiation medium. The proembryonal head formed in growth regulators free medium at 100 rpm. The embryonal head formed in basal medium containing 4% sucrose and 4 mg l -1 ABA in combination.
Page(s): 445-448
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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