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|Title:||Effects of genotype and culture medium on in vitro androgenesis in soybean (Glycine max Merr.)|
|Keywords:||Glycine max;anther culture;androgenesis;in vitro;genotype x medium interactions;haploid plants|
|IPC Code:||Int. Cl.7 A 01 H 4/00, 5/00|
|Abstract:||Anthers of ten genotypes of Glycine max were cultured on four fortified B5 media supplemented with different levels of growth hormones, viz. B5 DBIG (2.0 mg l-1 2,4-D+0.5 mg l-1 IBA+100.0 mg l-1 myo-Inositol+360.0 mg l-1 L-glutamine), B5 DB (2.0 mg l-1 2,4-D+0.5 mg l-1 BA), B5DK (2.0 mg l-1 2,4-D+0.5 mg l-1 Kinetin) and B5BKN (0.5 mg l-1 BA+0.5 mg l-1 Kinetin+1.0 mg l-1 NAA). All the media were supplemented with 90.0 g l-1 sucrose and 7.0 g l-1 agar. Significant differences in the response of genotypes, culture medium and genotype x medium interactions were observed for callus initiation, formation of morphogenic calli and plantlet regeneration. Genotype JS 90-41was found superior for in vitro androgenesis. Culture medium B5DBIG exhibited higher response for androgenic callus formation and haploid plant regeneration.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.03(3) [July 2004]|
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