|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | Effects of genotype and culture medium on in vitro androgenesis in soybean (Glycine max Merr.) |
| Authors: | Tiwari, Sharad
Shanker, Prem
Tripathi, Manoj |
| Keywords: | Glycine max
anther culture
androgenesis
in vitro
genotype x medium interactions
haploid plants |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | Anthers of ten genotypes of Glycine max were cultured on four fortified B5 media supplemented with different levels of growth hormones, viz. B5 DBIG (2.0 mg l-1 2,4-D+0.5 mg l-1 IBA+100.0 mg l-1 myo-Inositol+360.0 mg l-1 L-glutamine), B5 DB (2.0 mg l-1 2,4-D+0.5 mg l-1 BA), B5DK (2.0 mg l-1 2,4-D+0.5 mg l-1 Kinetin) and B5BKN (0.5 mg l-1 BA+0.5 mg l-1 Kinetin+1.0 mg l-1 NAA). All the media were supplemented with 90.0 g l-1 sucrose and 7.0 g l-1 agar. Significant differences in the response of genotypes, culture medium and genotype x medium interactions were observed for callus initiation, formation of morphogenic calli and plantlet regeneration. Genotype JS 90-41was found superior for in vitro androgenesis. Culture medium B5DBIG exhibited higher response for androgenic callus formation and haploid plant regeneration. |
| Page(s): | 441-444 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
|
|
