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Title: Effects of genotype and culture medium on in vitro androgenesis in soybean (Glycine max Merr.)
Authors: Tiwari, Sharad
Shanker, Prem
Tripathi, Manoj
Keywords: Glycine max
anther culture
androgenesis
in vitro
genotype x medium interactions
haploid plants
Issue Date: Jul-2004
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 4/00, 5/00
Abstract: Anthers of ten genotypes of Glycine max were cultured on four fortified B5 media supplemented with different levels of growth hormones, viz. B5 DBIG (2.0 mg l-1 2,4-D+0.5 mg l-1 IBA+100.0 mg l-1 myo-Inositol+360.0 mg l-1 L-glutamine), B5 DB (2.0 mg l-1 2,4-D+0.5 mg l-1 BA), B5DK (2.0 mg l-1 2,4-D+0.5 mg l-1 Kinetin) and B5BKN (0.5 mg l-1 BA+0.5 mg l-1 Kinetin+1.0 mg l-1 NAA). All the media were supplemented with 90.0 g l-1 sucrose and 7.0 g l-1 agar. Significant differences in the response of genotypes, culture medium and genotype x medium interactions were observed for callus initiation, formation of morphogenic calli and plantlet regeneration. Genotype JS 90-41was found superior for in vitro androgenesis. Culture medium B5DBIG exhibited higher response for androgenic callus formation and haploid plant regeneration.
Page(s): 441-444
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(3) [July 2004]

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