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Title: Selection of transformants of <i style="">Escherichia coli </i>containing cellulase gene from <i style="">Ruminococcus</i> <i style="">albus</i> isolated from rumen of crossbred steers
Authors: Chandrasekharaiah, M
Thulasi, A
Sampath, K T
Keywords: <i style="">Ruminococcus albus</i>
cellulase genes
<i style="">Escherichia coli</i>
Issue Date: Jul-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> C 12 N 15/10, 15/11
Abstract: <i style="">Ruminococcus albus</i>, considered best fibrolytic bacterium was isolated and characterized from the rumen of crossbred steers. It was found as wrinkled white colonies, slightly elevated with a slightly undulated margin, no surface spreading with the absence of liquefaction and a zone of hydrolysis. The cells were gram positive single cocci or diplococci. <i style="">R. albus</i> DNA was a high molecular weight DNA and it had just moved out of the well during electrophoresis. A genomic library of the Hind III fragments of <i style="">R. albus</i> DNA in pBR322 was constructed in <i style="">Escherichia coli</i>. Four clones were obtained with cellulase activity.
Description: 431-434
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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