Please use this identifier to cite or link to this item:
|Title:||Selection of transformants of Escherichia coli containing cellulase gene from Ruminococcus albus isolated from rumen of crossbred steers|
Sampath, K T
|Series/Report no.:||Int. Cl.7 C 12 N 15/10, 15/11|
|Abstract:||Ruminococcus albus, considered best fibrolytic bacterium was isolated and characterized from the rumen of crossbred steers. It was found as wrinkled white colonies, slightly elevated with a slightly undulated margin, no surface spreading with the absence of liquefaction and a zone of hydrolysis. The cells were gram positive single cocci or diplococci. R. albus DNA was a high molecular weight DNA and it had just moved out of the well during electrophoresis. A genomic library of the Hind III fragments of R. albus DNA in pBR322 was constructed in Escherichia coli. Four clones were obtained with cellulase activity.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.03(3) [July 2004]|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.