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Title: Selection of transformants of Escherichia coli containing cellulase gene from Ruminococcus albus isolated from rumen of crossbred steers
Authors: Chandrasekharaiah, M
Thulasi, A
Sampath, K T
Keywords: Ruminococcus albus
cellulase genes
transformants
Escherichia coli
rumen
Issue Date: Jul-2004
Publisher: CSIR
IPC CodeInt. Cl.7 C 12 N 15/10, 15/11
Abstract: Ruminococcus albus, considered best fibrolytic bacterium was isolated and characterized from the rumen of crossbred steers. It was found as wrinkled white colonies, slightly elevated with a slightly undulated margin, no surface spreading with the absence of liquefaction and a zone of hydrolysis. The cells were gram positive single cocci or diplococci. R. albus DNA was a high molecular weight DNA and it had just moved out of the well during electrophoresis. A genomic library of the Hind III fragments of R. albus DNA in pBR322 was constructed in Escherichia coli. Four clones were obtained with cellulase activity.
Page(s): 431-434
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(3) [July 2004]

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