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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | Selection of transformants of Escherichia coli containing cellulase gene from Ruminococcus albus isolated from rumen of crossbred steers |
| Authors: | Chandrasekharaiah, M
Thulasi, A
Sampath, K T |
| Keywords: | Ruminococcus albus
cellulase genes
transformants
Escherichia coli
rumen |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C 12 N 15/10, 15/11 |
| Abstract: | Ruminococcus albus, considered best fibrolytic bacterium was isolated and characterized from the rumen of crossbred steers. It was found as wrinkled white colonies, slightly elevated with a slightly undulated margin, no surface spreading with the absence of liquefaction and a zone of hydrolysis. The cells were gram positive single cocci or diplococci. R. albus DNA was a high molecular weight DNA and it had just moved out of the well during electrophoresis. A genomic library of the Hind III fragments of R. albus DNA in pBR322 was constructed in Escherichia coli. Four clones were obtained with cellulase activity. |
| Page(s): | 431-434 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
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