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Title: <i style="">In vitro</i> propagation of <i style="">Pterocarpus marsupium</i> Roxb.: An endangered medicinal tree
Authors: Tiwari, Sharad
Shah, Pankaj
Singh, Kanchan
Keywords: <i style="">in vitro</i> culture
nodal segments
plant regeneration
<i style="">Pterocarpus marsupium</i>
Issue Date: Jul-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 5/00
Abstract: Nodal segments of <i style="">Pterocarpus marsupium</i> Roxb. were inoculated on seven different media compositions, viz. MS, B5 and White’s without growth hormones (MS<sub>00 ,</sub> B5<sub>00 </sub>and WH<sub>00</sub>), each supplemented with 3.0 mg l<sup>-1</sup> BAP and 0.5 mg l<sup>-1</sup> NAA (MSBN, B5BN, WHBN) and MS media supplemented with 0.2 mg l<sup>-1</sup> IBA (MSIB). Seed germination improved in all the media studied, however, MS combinations were the best (95-100%). Maximum number of shoot induction per explant was in MS<sub>00</sub> (3.25) followed by MSIB (2.26). Maximum nodes per shootlet were observed in medium MSIB (4.95), while shoot length was maximum in MSIB (2.92 cm) followed by MS<sub>00</sub> (2.41). Regenerated plants were acclimatized and successfully transferred under field conditions.
Description: 422-425
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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