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Title: In vitro propagation of Pterocarpus marsupium Roxb.: An endangered medicinal tree
Authors: Tiwari, Sharad
Shah, Pankaj
Singh, Kanchan
Keywords: in vitro culture
nodal segments
plant regeneration
Pterocarpus marsupium
Issue Date: Jul-2004
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 4/00, 5/00
Abstract: Nodal segments of Pterocarpus marsupium Roxb. were inoculated on seven different media compositions, viz. MS, B5 and White’s without growth hormones (MS00 , B500 and WH00), each supplemented with 3.0 mg l-1 BAP and 0.5 mg l-1 NAA (MSBN, B5BN, WHBN) and MS media supplemented with 0.2 mg l-1 IBA (MSIB). Seed germination improved in all the media studied, however, MS combinations were the best (95-100%). Maximum number of shoot induction per explant was in MS00 (3.25) followed by MSIB (2.26). Maximum nodes per shootlet were observed in medium MSIB (4.95), while shoot length was maximum in MSIB (2.92 cm) followed by MS00 (2.41). Regenerated plants were acclimatized and successfully transferred under field conditions.
Page(s): 422-425
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(3) [July 2004]

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