|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | In vitro propagation of Pterocarpus marsupium Roxb.: An endangered medicinal tree |
| Authors: | Tiwari, Sharad
Shah, Pankaj
Singh, Kanchan |
| Keywords: | in vitro culture
nodal segments
plant regeneration
Pterocarpus marsupium |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/00 |
| Abstract: | Nodal segments of Pterocarpus marsupium Roxb. were inoculated on seven different media compositions, viz. MS, B5 and White’s without growth hormones (MS00 , B500 and WH00), each supplemented with 3.0 mg l-1 BAP and 0.5 mg l-1 NAA (MSBN, B5BN, WHBN) and MS media supplemented with 0.2 mg l-1 IBA (MSIB). Seed germination improved in all the media studied, however, MS combinations were the best (95-100%). Maximum number of shoot induction per explant was in MS00 (3.25) followed by MSIB (2.26). Maximum nodes per shootlet were observed in medium MSIB (4.95), while shoot length was maximum in MSIB (2.92 cm) followed by MS00 (2.41). Regenerated plants were acclimatized and successfully transferred under field conditions. |
| Page(s): | 422-425 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
|
|
