|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | Silver nitrate induced rooting and flowering in vitro on rare rhoeophytic woody medicinal plant, Rotula aquatica Lour. |
| Authors: | Chithra, M
Martin, K P
Sunandakumari, C
Madhusoodanan, P V |
| Keywords: | Rotula aquatica
liquid medium
plantlet survival
solid medium |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 H 4/00, 5/02, 5/06 |
| Abstract: | Silver nitrate induced enhanced rooting and flowering in vitro was achieved on Rotula aquatica L., a rare rhoeophytic woody aromatic medicinal plant. Solid and liquid nature of the medium, and growth regulators significantly influenced in vitro rooting and flowering. Half-strength MS liquid medium fortified with 2.69  M  -naphthaleneacetic acid (NAA) was effective among different auxins, which induced a mean of 7.3 roots per shoot. Plantlets acclimatized after auxin alone induced rooting exhibited 80% survival in field conditions. Nevertheless, the established plantlets exhibited 50% loss within 3 months. Addition of 11.7 M silver nitrate to half-strength MS liquid medium containing 2.69 M NAA increased the number of roots to 16.8 per shoot. Plantlets established after rooting in silver nitrate supplemented medium facilitated 95% survival, and the established plantlets did not exhibit loss even after 3 months. Dipping of the basal end of shoots developed in vitro in NAA (2.69 M) and silver nitrate (11.7 M) solution (made in water) for 25 days induced a mean of 12.4 roots per shoot. These plantlets transferred to small pots facilitated 90% survival. Half-strength MS liquid medium containing 2.69 M NAA and 11.7 M silver nitrate induced flowering earlier. The flowers were morphologically and structurally similar to that on plants growing in field conditions. |
| Page(s): | 418-421 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
|
|
